猪皮胶原蛋白提取过程中酶解条件优化及其结构鉴定
Optimization of Enzymolysis Conditions for Pigskin Collagen and Identification of Its Structure
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摘要: 采用酶法从猪皮中提取胶原蛋白，以胶原蛋白的提取率为评价指标，对工艺中的酶解pH ，酶解时间和胃蛋白酶用量3个因素进行优化，实验结果表明，酶解液pH2.2，酶解时间18h，酶用量1％为最佳工艺组合，在此条件下胶原蛋白提取率可达84.35％±0.33％，纯度为82.5％±1.6％；所得胶原主要由α，β和γ3种亚基组分组成，具有较高的热稳定性；红外光谱和圆二色谱测定结果显示，其具备典型的胶原蛋白吸收特征，证明胶原天然三螺旋结构保存完整．Abstract: Collagen in porcine skin was extracted with pepsin .With collagen extraction rate as the evalua‐tion indicator ,3 factors (enzyme solution pH ,enzymolysis time and dosage of pepsin ) of the extraction technology were optimized .The best processing combination was enzyme solution pH 2.2 + enzymolysis time 18 h + enzyme dosage 1% .Under such conditions ,a collagen extraction rate of 84.35% ± 0.33%was achieved ,with a collagen purity of 82.5% ± 1.6% .The collagen thus obtained was shown to compose mainly of α,βand γsubunits and to have fairly high thermal stability .Infrared spectroscopy and circular dichroism determination showed that it had the typical absorption characteristics of natural collagen ,and that its natural triple helix structure remained intact .
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