基于ISSR标记的44份桃种质资源遗传多样性分析
Study of Genetic Diversity of 44 Prunus persica Accessions Based on ISSR Markers
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摘要: 从100条ISSR引物中筛选出16条多态性高、重复性好的引物 ,对44份桃栽培品种进行遗传多样性分析 ,共扩增出141条带 ,其中120条带具有多态性 ,百分率高达85.1% ,每条引物扩增条带在4条到13条不等 ,平均8.8条 .44份材料的相似性系数在0.404~0.979之间 ,表明供试的44份桃品种间有较丰富的遗传多样性 .通过U PGM A聚类分析将桃资源材料分为5个亚组 .Abstract: In a study based on inter simple sequence repeat (ISSR) molecular markers ,16 primers were screened from 100 ISSR primers to analyze the genetic diversity of 44 Prunus persica accessions/cultivars . A total of 141 DNA fragments were amplified ,of which 120 were polymorphic ,accounting for 85.1% of the total .The number of DNA fragments amplified per primer ranged from 4 to 13 ,averaging 8.8 .The co-efficient of genetic similarity of the 44 P. persica cultivars ranged from 0.404 to 0.979 ,showing that there was rich genetic diversity among the cultivars .Based on UPGMA (unweighted pair-group method with arithmetic means) analysis ,the cultivars studied were clustered into five sub-groups .
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Key words:
- Prunus persica ISSR genetic diversity .
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