越橘"Legacy"离体培养及秋水仙素诱变研究初报
Tissue Culture of Vaccinium L. cv. "Legacy" and Mutagenic Effects of Colchicine
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摘要: 以越橘栽培品种"Legacy"茎段为外植体建立离体快繁体系后 ,研究了秋水仙素处理对"Legacy"组培苗的诱变影响 .结果表明 :4 ℃低温冷藏茎段24 h后 ,用0.15% HgCl2 消毒8 min ,可获得越橘"Legacy"无菌材料 .0.10%秋水仙素浸泡茎尖24 h诱变效果最佳 ,变异率达到10% ,变异株均为嵌合体 .0.05% 秋水仙素处理茎尖24 h后 ,继代后代增殖率与对照植株呈极显著差异 ,继代后代之间无显著性差异 ,其他越橘品种并没有表现出此现象 .Abstract: Plant regeneration system of cultivar "Legacy" of V accinium L. was established in an experi-ment ,in which the stem segments of the cultivar were used as explants and then colchicine immersion was made of the cultured plants for polyploidy induction .Aseptic plants were obtained by keeping the explants in cold storage (4 ℃) for 24 h and disinfecting them in 0.15% HgCl for 8 min .Soaking shoot tips for 24 h with 0.10% colchicine gave the best mutagenesis ,with a variation rate of 10% .The variants were all chi-meras .Soaking shoot tips for 24 h with 0.05% colchicine gave a multiplication rate of subculture signifi-cantly different from that of the control group .Such a phenomenon was not detected in other cultivars in this study .
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Key words:
- V accinium L.，in vitro culture，colchicine，polyploid /
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