4种贵州樱桃的高效离体再生

宋常美,晓鹏

doi:10.13718/j.cnki.xdzk.2015.09.003

西南大学学报（自然科学版）

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4种贵州樱桃的高效离体再生

宋常美,晓鹏. 4种贵州樱桃的高效离体再生[J]. 西南大学学报（自然科学版）, 2015, 37(9). doi: 10.13718/j.cnki.xdzk.2015.09.003

引用本文:

宋常美,晓鹏. 4种贵州樱桃的高效离体再生[J]. 西南大学学报（自然科学版）, 2015, 37(9). doi: 10.13718/j.cnki.xdzk.2015.09.003

SONG Chang-mei,WEN Xiao-peng. Highly Efficient in vit ro Regeneration of Four Cherry Lines in Guizhou via Organogenesis[J]. Journal of Southwest University Natural Science Edition, 2015, 37(9). doi: 10.13718/j.cnki.xdzk.2015.09.003

Citation:

SONG Chang-mei,WEN Xiao-peng. Highly Efficient in vit ro Regeneration of Four Cherry Lines in Guizhou via Organogenesis[J]. Journal of Southwest University Natural Science Edition, 2015, 37(9). doi: 10.13718/j.cnki.xdzk.2015.09.003

4种贵州樱桃的高效离体再生

宋常美,晓鹏

贵阳学院生物与环境工程学院,贵阳,550025 贵州大学贵州省农业生物工程重点实验室,贵阳,550025

Highly Efficient in vit ro Regeneration of Four Cherry Lines in Guizhou via Organogenesis

SONG Chang-mei,WEN Xiao-peng

摘要: 通过探讨植物生长调节剂对试管苗增殖、壮苗及生根的影响，建立了4种贵州樱桃的离体繁殖技术．结果表明：供试材料以MS＋1.5－2.0 mg／L 6‐BA＋0.5 mg／L IBA增殖效果良好，添加1.0 mg／L GA能明显促进增殖；添加0.5 mg／L GA利于壮苗；NAA最适合供试材料组培苗生根，沿河樱桃和凯里樱桃生根培养基为1／2 MS＋1.0 mg／L NAA ，赫章樱桃和红水樱桃为1／2 MS＋1.0 mg／L NAA＋0.5 mg／L GA ．
- 贵州 /
- 樱桃 /
- 植物生长调节剂 /
- 离体再生
Abstract: In the current work ,the influences of plant growth regulators on the multiplication ,growth vigor and root formation of test tube plantlets of four cherry ( Prunus pseudocerasu L.) lines from Guizhou Province were investigated ,and an optimized efficient in vitro regeneration system for them was established .The result showed that MS+1.5 -2.0 mg/L 6‐BA+0.5 mg/L IBA was best for bud multiplication of the tested lines , the addition of 1.0 mg/L GA significantly promoted multiplication ,and 0.5 mg/L GA was most suitable for the growth vigor of the in vitro shoots .NAA gave the best result for root formation .1/2MS+ 1.0 mg/L NAA was best for root formation of lines Yanhe and Kaili .However 1/2MS+1.0 mg/L NAA+0.5 mg/L GA was essential for root induction in lines Hezhang and Hongshui .
- Guizhou，Prunus pseudocerasu L.，plant growth regulator，in vitro regeneration /

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4种贵州樱桃的高效离体再生

宋常美,晓鹏

贵阳学院生物与环境工程学院,贵阳,550025 贵州大学贵州省农业生物工程重点实验室,贵阳,550025

关键词:

摘要: 通过探讨植物生长调节剂对试管苗增殖、壮苗及生根的影响，建立了4种贵州樱桃的离体繁殖技术．结果表明：供试材料以MS＋1.5－2.0 mg／L 6‐BA＋0.5 mg／L IBA增殖效果良好，添加1.0 mg／L GA能明显促进增殖；添加0.5 mg／L GA利于壮苗；NAA最适合供试材料组培苗生根，沿河樱桃和凯里樱桃生根培养基为1／2 MS＋1.0 mg／L NAA ，赫章樱桃和红水樱桃为1／2 MS＋1.0 mg／L NAA＋0.5 mg／L GA ．

English Abstract

Highly Efficient in vit ro Regeneration of Four Cherry Lines in Guizhou via Organogenesis

贵阳学院生物与环境工程学院,贵阳,550025 贵州大学贵州省农业生物工程重点实验室,贵阳,550025

Keywords:

Abstract: In the current work ,the influences of plant growth regulators on the multiplication ,growth vigor and root formation of test tube plantlets of four cherry ( Prunus pseudocerasu L.) lines from Guizhou Province were investigated ,and an optimized efficient in vitro regeneration system for them was established .The result showed that MS+1.5 -2.0 mg/L 6‐BA+0.5 mg/L IBA was best for bud multiplication of the tested lines , the addition of 1.0 mg/L GA significantly promoted multiplication ,and 0.5 mg/L GA was most suitable for the growth vigor of the in vitro shoots .NAA gave the best result for root formation .1/2MS+ 1.0 mg/L NAA was best for root formation of lines Yanhe and Kaili .However 1/2MS+1.0 mg/L NAA+0.5 mg/L GA was essential for root induction in lines Hezhang and Hongshui .