濒危植物珙桐种群遗传多样性分析

关萍,张玉晶,石建明,陈业

doi:10.13718/j.cnki.xdzk.2015.09.011

西南大学学报（自然科学版）

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濒危植物珙桐种群遗传多样性分析

关萍,张玉晶,石建明,陈业. 濒危植物珙桐种群遗传多样性分析[J]. 西南大学学报（自然科学版）, 2015, 37(9). doi: 10.13718/j.cnki.xdzk.2015.09.011

引用本文:

关萍,张玉晶,石建明,陈业. 濒危植物珙桐种群遗传多样性分析[J]. 西南大学学报（自然科学版）, 2015, 37(9). doi: 10.13718/j.cnki.xdzk.2015.09.011

GUAN Ping,ZHANG Yu-jing,SHI Jian-ming,CHEN Ye. ISSR Analysis of the Genetic Diversity of the Endangered Plant Davidia involucrate Baill[J]. Journal of Southwest University Natural Science Edition, 2015, 37(9). doi: 10.13718/j.cnki.xdzk.2015.09.011

Citation:

GUAN Ping,ZHANG Yu-jing,SHI Jian-ming,CHEN Ye. ISSR Analysis of the Genetic Diversity of the Endangered Plant Davidia involucrate Baill[J]. Journal of Southwest University Natural Science Edition, 2015, 37(9). doi: 10.13718/j.cnki.xdzk.2015.09.011

濒危植物珙桐种群遗传多样性分析

关萍,张玉晶,石建明,陈业

贵州大学生命科学学院,贵阳,550025

ISSR Analysis of the Genetic Diversity of the Endangered Plant Davidia involucrate Baill

GUAN Ping,ZHANG Yu-jing,SHI Jian-ming,CHEN Ye

摘要: 采用ISSR分子标记技术，对珙桐6个种群和光叶珙桐1个种群共117份样品进行遗传多样性分析．结果表明：用12条引物对117份样品进行扩增，共检测扩增位点199个，其中多态性位点177个．在物种水平上，多态性位点百分率（PPL）为88.94％，Nei's基因多样性（H）为0.2784，shannon信息指数（I）为0.4187．种群水平的多态性相对较低，多态百分率在35.18％～48.74％之间，Nei's基因多样性（H ）为0.1260～0.1795，shannon信息指数（I）为0.1881～0.2651．基于Nei's遗传多样性分析得出的种群间遗传分化系数（Gst）为0.4745，表明有47.45％的遗传变异存在于种群间，而种群内的遗传变异占总变异的52.55％，种群间基因流（Nm）为0.5537．Mantel检测显示，种群间的遗传距离和地理距离之间不存在显著的相关性．
- 珙桐 /
- ISSR /
- 遗传多样性
Abstract: ISSR (inter‐simple sequence repeat) markers were used to analyze the genetic diversity of 6 pop‐ulations of Davidia involucrate Baill and 1 population of D. involucrata Baill. var. vilmoriniana (Dode) Wanger ,the samples totaling 117 .A total of 199 discernible DNA loci were generated by 12 ISSR primers , of which 177 were polymorphic .At the species level ,percentage of polymorphic loci (PPL) ,genetic diver‐sity (He) and Shannon information index (I) was 88.94% ,0.278 4 and 0.418 7 ,respectively Polymor‐phism at the population level was rather low ,PPL ranging from 35.18% to 48.74% ,He from 0.126 0 to 0.179 5 ,and I from 0.188 1 to 0.265 1 .The genetic differentiation (Gst) based on the Nei's genetic diver‐sity was 0.474 5 ,indicating that there was 47.45 % of the variance among them and 52.55 % of the vari‐ance within them ,and the gene flow was 0.553 7 among them .Cluster analysis showed that there was no significant correlation between genetic distance and geographic distance .
- Davidia involucrate Baill.，ISSR，genetic diversity /

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濒危植物珙桐种群遗传多样性分析

关萍,张玉晶,石建明,陈业

贵州大学生命科学学院,贵阳,550025

关键词:

珙桐 /
ISSR /
遗传多样性

摘要: 采用ISSR分子标记技术，对珙桐6个种群和光叶珙桐1个种群共117份样品进行遗传多样性分析．结果表明：用12条引物对117份样品进行扩增，共检测扩增位点199个，其中多态性位点177个．在物种水平上，多态性位点百分率（PPL）为88.94％，Nei's基因多样性（H）为0.2784，shannon信息指数（I）为0.4187．种群水平的多态性相对较低，多态百分率在35.18％～48.74％之间，Nei's基因多样性（H ）为0.1260～0.1795，shannon信息指数（I）为0.1881～0.2651．基于Nei's遗传多样性分析得出的种群间遗传分化系数（Gst）为0.4745，表明有47.45％的遗传变异存在于种群间，而种群内的遗传变异占总变异的52.55％，种群间基因流（Nm）为0.5537．Mantel检测显示，种群间的遗传距离和地理距离之间不存在显著的相关性．

English Abstract

ISSR Analysis of the Genetic Diversity of the Endangered Plant Davidia involucrate Baill

贵州大学生命科学学院,贵阳,550025

Keywords:

珙桐 /
ISSR /
遗传多样性

Abstract: ISSR (inter‐simple sequence repeat) markers were used to analyze the genetic diversity of 6 pop‐ulations of Davidia involucrate Baill and 1 population of D. involucrata Baill. var. vilmoriniana (Dode) Wanger ,the samples totaling 117 .A total of 199 discernible DNA loci were generated by 12 ISSR primers , of which 177 were polymorphic .At the species level ,percentage of polymorphic loci (PPL) ,genetic diver‐sity (He) and Shannon information index (I) was 88.94% ,0.278 4 and 0.418 7 ,respectively Polymor‐phism at the population level was rather low ,PPL ranging from 35.18% to 48.74% ,He from 0.126 0 to 0.179 5 ,and I from 0.188 1 to 0.265 1 .The genetic differentiation (Gst) based on the Nei's genetic diver‐sity was 0.474 5 ,indicating that there was 47.45 % of the variance among them and 52.55 % of the vari‐ance within them ,and the gene flow was 0.553 7 among them .Cluster analysis showed that there was no significant correlation between genetic distance and geographic distance .