转几丁质酶基因烟草对三种真菌拮抗作用的研究

闫文慧,王兵,楼康怡,杨星勇,申鸿

doi:10.13718/j.cnki.xsxb.2014.10.014

西南师范大学学报(自然科学版)

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转几丁质酶基因烟草对三种真菌拮抗作用的研究

闫文慧,王兵,楼康怡,杨星勇,申鸿. 转几丁质酶基因烟草对三种真菌拮抗作用的研究[J]. 西南师范大学学报(自然科学版), 2014, 39(10). doi: 10.13718/j.cnki.xsxb.2014.10.014

引用本文:

YAN Wen-hui,WANG Bing,LOU Kang-yi,YANG Xing-yong,SHEN Hong. On Antagonistic Effect of Transgenic Tobacco Transformed with Chitinase Gene (T-Chit) on Three Species of Fungi[J]. Journal of Southwest China Normal University(Natural Science Edition), 2014, 39(10). doi: 10.13718/j.cnki.xsxb.2014.10.014

Citation:

YAN Wen-hui,WANG Bing,LOU Kang-yi,YANG Xing-yong,SHEN Hong. On Antagonistic Effect of Transgenic Tobacco Transformed with Chitinase Gene (T-Chit) on Three Species of Fungi[J]. Journal of Southwest China Normal University(Natural Science Edition), 2014, 39(10). doi: 10.13718/j.cnki.xsxb.2014.10.014

转几丁质酶基因烟草对三种真菌拮抗作用的研究

闫文慧,王兵,楼康怡,杨星勇,申鸿

西南大学资源环境学院,重庆,400716 重庆师范大学生命科学学院,重庆,401331

On Antagonistic Effect of Transgenic Tobacco Transformed with Chitinase Gene (T-Chit) on Three Species of Fungi

YAN Wen-hui,WANG Bing,LOU Kang-yi,YANG Xing-yong,SHEN Hong

摘要: 几丁质酶广泛存在于高等植物体内，它可以分解真菌细胞壁中的几丁质，破坏其细胞结构，从而获得对真菌的抗性。试验以转苦瓜几丁质酶基因烟草（T-Chit）为供试植物，测定植株根系和叶片内几丁质酶活性，选取了3种具有代表性的真菌：毛霉、木霉、禾谷镰刀霉，通过抑菌试验验证了T-Chit组织萃取液对真菌的抗性。结果表明：1）T-Chit几丁质酶活性显著高于非转基因烟草（Nt-X），且转基因烟草根系几丁质酶活性比叶片高86％；2）T-Chit组织萃取液对毛霉生长具有明显抑制作用，根系强于叶片；3）T-Chit对木霉和禾谷镰刀霉的抑制具有时空效应：抑菌效果与植株部位及作用时间有关。
- 转几丁质酶基因烟草 /
- 几丁质酶活性 /
- 萃取液 /
- 真菌
Abstract: Chitinase widely exists in higher plants .It is able to degrade chitin in the cell walls of fungi , thus destroying the fungal cell structure .Depending on this functional mechanism ,plants gain resistance to fungi .In this study , transgenic tobacco has been transformed with chitinase gene (T-Chit ) of bal-sampear as experimental plants ,and we have measured the chitinase activity in roots and leaves ,and dem-onstrated the resistance of T-Chit tissue extract against Mucor racemosus sp ., Trichoderma viride sp ., and Fusarium graminearum sp .The results show that (1) Chitinase activity of T-Chit is greater than pa-rental tobacco plants (Nt-X) ,and in transgenic tobacco ,chitinase activity in root is 86% higher than that in leaf blade ;(2) the extract of T-Chit has strong antifungal effects against Mucor racemosus sp .,and re-sistance of root system is stronger than that of leaf blade ;(3) the resistance of T-Chit tissue extract has space-time effect on Trichoderma viride sp .and Fusarium graminearum sp .,and the effect varies with plant sites and action time .
- T-Chit，chitinase activity，tissue extract，fungi /

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转几丁质酶基因烟草对三种真菌拮抗作用的研究

闫文慧,王兵,楼康怡,杨星勇,申鸿

西南大学资源环境学院,重庆,400716 重庆师范大学生命科学学院,重庆,401331

关键词:

摘要: 几丁质酶广泛存在于高等植物体内，它可以分解真菌细胞壁中的几丁质，破坏其细胞结构，从而获得对真菌的抗性。试验以转苦瓜几丁质酶基因烟草（T-Chit）为供试植物，测定植株根系和叶片内几丁质酶活性，选取了3种具有代表性的真菌：毛霉、木霉、禾谷镰刀霉，通过抑菌试验验证了T-Chit组织萃取液对真菌的抗性。结果表明：1）T-Chit几丁质酶活性显著高于非转基因烟草（Nt-X），且转基因烟草根系几丁质酶活性比叶片高86％；2）T-Chit组织萃取液对毛霉生长具有明显抑制作用，根系强于叶片；3）T-Chit对木霉和禾谷镰刀霉的抑制具有时空效应：抑菌效果与植株部位及作用时间有关。

English Abstract

On Antagonistic Effect of Transgenic Tobacco Transformed with Chitinase Gene (T-Chit) on Three Species of Fungi

西南大学资源环境学院,重庆,400716 重庆师范大学生命科学学院,重庆,401331

Keywords:

Abstract: Chitinase widely exists in higher plants .It is able to degrade chitin in the cell walls of fungi , thus destroying the fungal cell structure .Depending on this functional mechanism ,plants gain resistance to fungi .In this study , transgenic tobacco has been transformed with chitinase gene (T-Chit ) of bal-sampear as experimental plants ,and we have measured the chitinase activity in roots and leaves ,and dem-onstrated the resistance of T-Chit tissue extract against Mucor racemosus sp ., Trichoderma viride sp ., and Fusarium graminearum sp .The results show that (1) Chitinase activity of T-Chit is greater than pa-rental tobacco plants (Nt-X) ,and in transgenic tobacco ,chitinase activity in root is 86% higher than that in leaf blade ;(2) the extract of T-Chit has strong antifungal effects against Mucor racemosus sp .,and re-sistance of root system is stronger than that of leaf blade ;(3) the resistance of T-Chit tissue extract has space-time effect on Trichoderma viride sp .and Fusarium graminearum sp .,and the effect varies with plant sites and action time .