手性膦酸酯硫脲的抗TMV活性及作用机制研究

刘静姿,胡俊锋,史大斌,宋宝安,吴剑

doi:10.13718/j.cnki.xsxb.2015.01.008

西南师范大学学报(自然科学版)

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手性膦酸酯硫脲的抗TMV活性及作用机制研究

刘静姿,胡俊锋,史大斌,宋宝安,吴剑. 手性膦酸酯硫脲的抗TMV活性及作用机制研究[J]. 西南师范大学学报(自然科学版), 2015, 40(1). doi: 10.13718/j.cnki.xsxb.2015.01.008

引用本文:

LIU Jing-zi,HU Jun-feng,SHI Da-bin,SONG Bao-an,WU Jian. On Antiviral Activity and Functional Mechanism of Chiral Thioureas Containing Phosphonate Moiety[J]. Journal of Southwest China Normal University(Natural Science Edition), 2015, 40(1). doi: 10.13718/j.cnki.xsxb.2015.01.008

Citation:

LIU Jing-zi,HU Jun-feng,SHI Da-bin,SONG Bao-an,WU Jian. On Antiviral Activity and Functional Mechanism of Chiral Thioureas Containing Phosphonate Moiety[J]. Journal of Southwest China Normal University(Natural Science Edition), 2015, 40(1). doi: 10.13718/j.cnki.xsxb.2015.01.008

手性膦酸酯硫脲的抗TMV活性及作用机制研究

刘静姿,胡俊锋,史大斌,宋宝安,吴剑

贵阳医学院,贵阳,550004 遵义医学院,贵州遵义,563000 贵州大学精细化工研究开发中心/教育部绿色农药与生物工程重点实验室,贵阳,550025

On Antiviral Activity and Functional Mechanism of Chiral Thioureas Containing Phosphonate Moiety

LIU Jing-zi,HU Jun-feng,SHI Da-bin,SONG Bao-an,WU Jian

摘要: 采用半叶枯斑法，测定了手性膦酸酯硫脲化合物的抗烟草花叶病毒（TMV）活性，化合物Ⅰ3和Ⅰ14在体积质量分数为500 mg／L时对TMV的治疗效率分别为53.2％和56.7％，接近或超过对照药剂宁南霉素（治疗效率为54.3％）；通过抗黄瓜花叶病毒（CMV）活性筛选还发现，Ⅰ3和Ⅰ14对CMV的治疗效率分别为53.4％和54.6％，接近对照药宁南霉素（56.3％）．在化合物Ⅰ3的作用下，研究了烟草叶片中叶绿素质量分数和3种防御酶（PAL ， POD和SOD）活性的变化趋势，结果表明经化合物Ⅰ3处理后烟草植株体内能产生与抗病机制相关的信号，从而启动植株体内一系列的抗病因子，促进烟草体内防御酶系产生，增强了植物的系统抗病性而达到抗TMV的效果．同时，通过分子对接考察了化合物Ⅰ3和Ⅰ14作用于病毒大分子T M V可能的活性靶标，说明氢键在抗病毒活性中发挥重要的作用．
- 手性硫脲 /
- 膦酸酯 /
- 抗TMV活性 /
- 作用机制
Abstract: A half-leaf method has been used to determine the in vivo curative ,protective and inactivation ef-ficacies of title products anti-TMV(or CMV) at the concentration of 0 .5 mg/mL .The results show that compounds Ⅰ 3 and Ⅰ 14 possess good anti-TMV and anti-CMV activity ,the anti-TMV curative rates are 53 .2% and 56 .7% ,respectively ;and anti-CM V curative rates are 53 .4% and 54 .6% ,respectively ,w hich are near or exceed that of the commercial control Ningnanmycin .In enzyme activity method ,chlorophyll , PAL ,POD ,SOD contents in Ⅰ 3-treated tobacco show some definite trend within a certain period of time . It has been found that compound Ⅰ 3 could increase the chlorophyll content of tobacco leaves and the activi-ty of three defense enzymes such as PAL ,POD and SOD .Furthermore ,the targets of anti-TMV activities possibly have been discovered with compounds Ⅰ 3 (or Ⅰ 14 ) and TMV by molecular docking .
- chiral thiourea，phosphonate，anti-T M V activities，functional mechanism /

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手性膦酸酯硫脲的抗TMV活性及作用机制研究

刘静姿,胡俊锋,史大斌,宋宝安,吴剑

贵阳医学院,贵阳,550004 遵义医学院,贵州遵义,563000 贵州大学精细化工研究开发中心/教育部绿色农药与生物工程重点实验室,贵阳,550025

关键词:

摘要: 采用半叶枯斑法，测定了手性膦酸酯硫脲化合物的抗烟草花叶病毒（TMV）活性，化合物Ⅰ3和Ⅰ14在体积质量分数为500 mg／L时对TMV的治疗效率分别为53.2％和56.7％，接近或超过对照药剂宁南霉素（治疗效率为54.3％）；通过抗黄瓜花叶病毒（CMV）活性筛选还发现，Ⅰ3和Ⅰ14对CMV的治疗效率分别为53.4％和54.6％，接近对照药宁南霉素（56.3％）．在化合物Ⅰ3的作用下，研究了烟草叶片中叶绿素质量分数和3种防御酶（PAL ， POD和SOD）活性的变化趋势，结果表明经化合物Ⅰ3处理后烟草植株体内能产生与抗病机制相关的信号，从而启动植株体内一系列的抗病因子，促进烟草体内防御酶系产生，增强了植物的系统抗病性而达到抗TMV的效果．同时，通过分子对接考察了化合物Ⅰ3和Ⅰ14作用于病毒大分子T M V可能的活性靶标，说明氢键在抗病毒活性中发挥重要的作用．

English Abstract

On Antiviral Activity and Functional Mechanism of Chiral Thioureas Containing Phosphonate Moiety

贵阳医学院,贵阳,550004 遵义医学院,贵州遵义,563000 贵州大学精细化工研究开发中心/教育部绿色农药与生物工程重点实验室,贵阳,550025

Keywords:

Abstract: A half-leaf method has been used to determine the in vivo curative ,protective and inactivation ef-ficacies of title products anti-TMV(or CMV) at the concentration of 0 .5 mg/mL .The results show that compounds Ⅰ 3 and Ⅰ 14 possess good anti-TMV and anti-CMV activity ,the anti-TMV curative rates are 53 .2% and 56 .7% ,respectively ;and anti-CM V curative rates are 53 .4% and 54 .6% ,respectively ,w hich are near or exceed that of the commercial control Ningnanmycin .In enzyme activity method ,chlorophyll , PAL ,POD ,SOD contents in Ⅰ 3-treated tobacco show some definite trend within a certain period of time . It has been found that compound Ⅰ 3 could increase the chlorophyll content of tobacco leaves and the activi-ty of three defense enzymes such as PAL ,POD and SOD .Furthermore ,the targets of anti-TMV activities possibly have been discovered with compounds Ⅰ 3 (or Ⅰ 14 ) and TMV by molecular docking .