摘要:
为探讨长期有氧运动对年老大鼠骨组织中Wnt/β-catenin通路的影响, 将实验分为: 青年组(Young), 年老大鼠组(Old), 年老大鼠+运动组(Old+EX), 运动为跑台运动, 持续12周. 结果发现: 与青年组大鼠相比, 老年组大鼠的股骨和L2-5段椎骨的骨密度(BMD)明显下降, 股骨的极限载荷和刚度明显降低;年老大鼠经过长期有氧运动后, 股骨和L2-5段椎骨的骨密度、 股骨的极限载荷和刚度都得到明显增加. 实时定量PCR结果显示老年组大鼠的股骨中Wnt1, Wnt3a, Lrp5, Axin2和ctnnb1的mRNA水平明显降低, 但长期进行有氧运动的老年组大鼠股骨中Wnt1, Wnt3a, Lrp5, Axin2和ctnnb1的mRNA水平得到明显增加. 可以认为长期有氧运动可上调老年大鼠骨组织中Wnt/β-catenin通路, 有助于减轻或延缓年老大鼠的骨质丢失.
Abstract:
The aim of this paper is to investigate the role of Wnt/β-catenin pathway in chronic aerobic exercise-induced attenuation of osteoporosis in aged rats.Male rats have randomly been divided into three groups as follows: 1) sedentary young group (Young, as control group);2) sedentary old group (Old);and 3) exercised-trained old group (Old+EX).Exercise for the treadmill exercise for 12 weeks.In this work, chronic aerobic exercise training in aged rats prevented the reduction of bone mineral density of femurs and L2-5 vertebra and preserved mechanical strength including ultimate load and stiffness in femurs.Interestingly, chronic aerobic exercise in aged rats unregulated mRNA expression of Wnt1, Wnt3a, Lrp5, Axin2, and ctnnb1 in femurs.It is concluded that chronic aerobic exercise training ameliorated senior osteoporosis in aged rats via activating Wnt/β-catenin pathway, at least in part.
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