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辣椒青枯病罹病与健康植株根际土壤微生物群落多样性研究

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刘烈花, 董鹏, 况觅, 等. 辣椒青枯病罹病与健康植株根际土壤微生物群落多样性研究[J]. 植物医学, 2021, (1): 41-47. doi: 10.13718/j.cnki.zwys.2021.01.008
引用本文: 刘烈花, 董鹏, 况觅, 等. 辣椒青枯病罹病与健康植株根际土壤微生物群落多样性研究[J]. 植物医学, 2021, (1): 41-47. doi: 10.13718/j.cnki.zwys.2021.01.008
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LIU Lie-hua, DONG Peng, KUANG Mi, et al. Diversity Analysis of Soil Microbial Community in the Rhizosphere Soil of Bacterial Wilt-Diseased and Healthy Pepper Plants[J]. PLANT HEALTH AND MEDICINE, 2021, (1): 41-47. doi: 10.13718/j.cnki.zwys.2021.01.008
Citation: LIU Lie-hua, DONG Peng, KUANG Mi, et al. Diversity Analysis of Soil Microbial Community in the Rhizosphere Soil of Bacterial Wilt-Diseased and Healthy Pepper Plants[J]. PLANT HEALTH AND MEDICINE, 2021, (1): 41-47. doi: 10.13718/j.cnki.zwys.2021.01.008
shu

辣椒青枯病罹病与健康植株根际土壤微生物群落多样性研究

  • 1. 西南大学 植物保护学院, 重庆 400715;

  • 2. 重庆市农业技术推广总站, 重庆 401121;

  • 3. 重庆安邦农业发展有限公司, 重庆 400030

详细信息
    作者简介:

    刘烈花,硕士研究生,主要从事天然产物农药研究.E-mail:llh199510@email.swu.edu.cn .

  • 中图分类号: S436.418.1

Diversity Analysis of Soil Microbial Community in the Rhizosphere Soil of Bacterial Wilt-Diseased and Healthy Pepper Plants

  • 1. School of Plant Protection, Southwest University, Chongqing 400715, China;

  • 2. Chongqing General Station of Agricultural Technology Extension, Chongqing 401121, China;

  • 3. Chongqing Anbang Agricultural Development Co., Ltd, Chongqing 400030, China

  • 摘要: 系统研究和分析辣椒青枯病常发地发病与健康植株土壤微生物群落结构特征，为辣椒青枯病的绿色防治提供理论依据.基于16S rDNA基因高通量测序技术，对辣椒青枯病发病和健康植株根际土壤微生物群落结构和组成进行分析，同时采用biologyeco平板培养技术研究其土壤微生物群落代谢多样性和功能多样性的特征.结果表明，辣椒青枯病发病和健康植株根际土壤微生物群落组成之间存在显著差异，辣椒青枯病发病土壤的OTU为4 566个，辣椒青枯病健康土壤的OTU为4 167个.依据OTU所属细菌物种信息对土壤细菌群落结构进行分析，变形菌门在发病和健康土壤中均为优势细菌类群，其次为放线菌门类群.其中健康植株根际土壤中芽单胞菌门（Gemmatimonadetes）、装甲菌门（Armatimonadetes）的相对丰度比发病植株的分别高出了4.37，3.87倍，而发病植株根际土壤中厚壁菌门（Firmicutes）的相对丰度比健康植株的高出了3.87倍.辣椒青枯病发病土壤和健康土壤的土壤微生物代谢多样性也存在显著差异，同时，健康土壤中其微生物群落代谢得到显著增强，特别是对酚类化合物的利用显著增多，对辣椒抗病性存在显著的影响.研究表明，辣椒青枯病发病和健康植株根际土壤微生物群落组成和结构之间存在显著差异，并且健康土壤的微生物群落对酚类化合物的利用显著增强.
    Abstract: In order to provide a theoretical basis for the green control of pepper bacterial wilt, a study was made in which the characteristics of soil microbial community structure of pepper bacterial wilt-affected and healthy pepper plants in the areas where this disease frequently occurs were systematically analyzed. Based on the 16S rDNA gene high-throughput sequencing technology, the structure and composition of the soil microbial community in the rhizosphere of pepper bacterial wilt-diseased and healthy plants were compared, and the biology eco plate culture technology was used to study the characteristics of metabolic diversity and function diversityof the soil microbial community. A significant difference was detected between diseased plants and healthy plants in the composition of rhizosphere soil microbial community, the OTU (operational taxonomic unit) of the soil affected by pepper bacterial wilt being 4 566, and that of the healthy soil being 4 167. According to the bacterial species information of OTU, the soil bacterial community structure was analyzed. Proteobacteria were found to be the dominant bacterial groups in diseased and healthy soil, followed by Actinomycetes. Among them, the relative abundance of Gemmatimonadetes and Armatimonadetes in the rhizosphere soil of healthy plants was 4.37 and 3.87 times higher than that of diseased plants, respectively, while the relative abundance of Firmicutes was 3.87 times higher in the rhizosphere soil of diseased plants than that of healthy plants. Significant differences also existed in the diversity of soil microbial metabolism between pepper bacterial wilt-affected soil and healthy soil. At the same time, the metabolism of the microbial community in healthy soil was found to be significantly enhanced, especially the use of phenolic compounds increased significantly, which hada significant impact on disease resistance of pepper plants.
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  • [1] 邢玉晓, 刘方菁, 丁涌波, 等. 石柱主栽朝天红辣椒腌制加工适性研究[J]. 食品工业科技, 2015, 36(12):113-121.
    [2] 江欢欢, 程凯, 杨兴明, 等. 辣椒青枯病拮抗菌的筛选及其生物防治效应[J]. 土壤学报, 2010, 47(6):1225-1231.
    [3] 向立刚, 周浩, 汪汉成, 等. 健康与感染青枯病烟株根际土壤与茎秆细菌群落结构与多样性[J]. 微生物学报, 2019, 59(10):1984-1999.
    [4] 丁伟, 刘晓姣. 植物医学的新概念——生物屏障[J]. 植物医生, 2019, 32(1):1-6.
    [5] MIHO S, NAKAJIMA Y, MMASUYA K, et al. Comparison of Phospholipid Fatty Acid Composition in Percolating Water, Floodwater, and the Plow Layer Soil During the Rice Cultivation Period in a Japanese Paddy Field[J]. Soil Science and Plant Nutrition, 2002, 48(4):595-600.
    [6] 郑雪芳, 刘波, 朱育菁. 青枯病植物疫苗对番茄根系土壤微生物群落结构的影响[J]. 中国生物防治学报, 2017, 33(3):385-393.
    [7] KERRI L S, JACKSON L E, CALDERON F J, et al. Soil Microbial Community Composition and Land Use History in Cultivated and Grassland Ecosystems of Coastal California[J]. Soil Biology &Biochemistry, 2002, 34(11):1599-1611.
    [8] 蔡燕飞, 廖宗文, 章家恩, 等. 生态有机肥对番茄青枯病及土壤微生物多样性的影响[J]. 应用生态学报, 2003, 14(3):349-353.
    [9] 马云华, 魏珉, 王秀峰. 日光温室连作土壤酚类物质变化及其对黄瓜根系抗病性相关酶的影响[J]. 应用生态学报, 2005, 16(1):79-82.
    [10] 林开敏, 叶发茂, 林艳, 等. 酚类物质对土壤和植物的作用机制研究进展[J]. 中国生态农业学报, 2010, 18(5):1130-1137.
    [11] 韩树全. 酚类物质抗香蕉枯萎病的作用机理[D]. 武汉:华中农业大学, 2014.
    [12] HARSH PB, PRITHIVIRAJ B, JHA A K, et al. Mediation of Pathogen Resistance by Exudation of Antimicrobials from Roots[J]. Nature, 2005, 434(7030):217-221.
    [13] LANOUE A, BURLATV, HENKESG J, et al. De Novo Biosynthesis of Defense Root Exudates in Response to Fusarium Attack in Barley[J]. The New Phytologist, 2010, 185(2):577-588.
    [14] ZHOU X, WU F.p-Coumaric Acid Influenced Cucumber Rhizosphere Soil Microbial Communities and the Growth of Fusarium oxysporum f.sp. cucumerinum Owen[J].PLoS ONE, 2012, 7(10):e48288.
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出版历程
  • 收稿日期:  2020-12-16

辣椒青枯病罹病与健康植株根际土壤微生物群落多样性研究

    作者简介: 刘烈花,硕士研究生,主要从事天然产物农药研究.E-mail:llh199510@email.swu.edu.cn
  • 1. 西南大学 植物保护学院, 重庆 400715;
  • 2. 重庆市农业技术推广总站, 重庆 401121;
  • 3. 重庆安邦农业发展有限公司, 重庆 400030
  • 收稿日期:  2020-12-16
基金项目: 

摘要: 系统研究和分析辣椒青枯病常发地发病与健康植株土壤微生物群落结构特征，为辣椒青枯病的绿色防治提供理论依据.基于16S rDNA基因高通量测序技术，对辣椒青枯病发病和健康植株根际土壤微生物群落结构和组成进行分析，同时采用biologyeco平板培养技术研究其土壤微生物群落代谢多样性和功能多样性的特征.结果表明，辣椒青枯病发病和健康植株根际土壤微生物群落组成之间存在显著差异，辣椒青枯病发病土壤的OTU为4 566个，辣椒青枯病健康土壤的OTU为4 167个.依据OTU所属细菌物种信息对土壤细菌群落结构进行分析，变形菌门在发病和健康土壤中均为优势细菌类群，其次为放线菌门类群.其中健康植株根际土壤中芽单胞菌门（Gemmatimonadetes）、装甲菌门（Armatimonadetes）的相对丰度比发病植株的分别高出了4.37，3.87倍，而发病植株根际土壤中厚壁菌门（Firmicutes）的相对丰度比健康植株的高出了3.87倍.辣椒青枯病发病土壤和健康土壤的土壤微生物代谢多样性也存在显著差异，同时，健康土壤中其微生物群落代谢得到显著增强，特别是对酚类化合物的利用显著增多，对辣椒抗病性存在显著的影响.研究表明，辣椒青枯病发病和健康植株根际土壤微生物群落组成和结构之间存在显著差异，并且健康土壤的微生物群落对酚类化合物的利用显著增强.

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