Study on Effect of Different Application Methods on Control of Tobacco Root-knot Nematode Disease
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摘要: 根结线虫病是烟草上一种重要的土传病害,目前烟叶生产上亟需有效的病害防控方法.本研究通过田间小区试验探究两种药剂的3种不同施用方式对烟草根结线虫病的影响效果,旨在明确不同药剂防控烟草根结线虫病的较优施药方式,以提高防治效率和效果、维护生态环境.结果表明,两种药剂的3种不同施用方式对烟草根结线虫病均有较好的防控效果,阿维菌素受施用方式影响较小,移栽窝施或灌根处理对烟草根结线虫的防效均较佳,约为53.00%~71.06%;厚孢轮枝菌受施用方式影响较大,移栽灌根处理对烟草根结线虫病的防效可达53.27%,但移栽窝施处理防效仅为15.83%.因此,针对降雨少、土壤含水量低的烟区,采用微生物菌剂防控烟草根结线虫病时,可选择灌根窝施或施用后应及时补充土壤水分的处理方式,以提高药剂/菌剂利用率和病害防控效果,而化学药剂施用后也应及时补充土壤水分,实现药剂的减量增效.本研究为根结线虫病的防控提供了有效参考.Abstract: Tobacco root-knot nematode is one of the most important soil-born tobacco diseases in southwest of China, especially in Liangshan area of Sichuan Province. In this research, we evaluated the effects of three kinds of application methods with two kinds of agents on control of tobacco root-knot nematode disease with different treatment time in the field. The purpose of this study was to clarify the optimal application methods for different pesticides to prevent and control tobacco root-knot nematode, so as to improve the control efficiency and effect, and maintain the ecological environment. The results showed that all kinds of application methods with two agents could control the occurrence of tobacco nematode disease and promote the growth of tobacco plants. The control efficiency of abamectin was not obviously affected by the application method. The control efficiencies were ranged from 53.00%~71.06%. The control efficiency of Verticillium chlamydosporium was significantly affected by the application method. The control efficiencies by root-irrigation and pit application were 53.27% and 15.83%. Therefore, we suggested that for areas with less rainfall and low soil water content, control of tobacco root knot nematode disease by microbial agents should supplement the water in time to improve the utilization of agents and the effect of disease control. After the application of chemical agents, soil water should be replenished in time to reduce the usage of chemical and increase the efficiency. This study could provide an effective reference for the control of root knot nematode disease.
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Key words:
- tobacco root-knot nematodes /
- disease control /
- chemical control /
- bio-control /
- application methods .
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[1] 崔江宽,任豪豪,孟颢光,等.我国烟草根结线虫病发生与防治研究进展[J].植物病理学报,2021,51(5):663-682. [2] 张宗锦,闫芳芳,孔垂旭,等.烤烟菽麻间作对烟草根结线虫防效及烟叶产质量的影响[J].中国烟草科学,2019,40(2):52-56. [3] 徐兴阳,杨艳梅,端永明,等.昆明烤烟种植区根结线虫种类的初步鉴定[J].云南农业大学学报(自然科学),2017,32(5):947-951. [4] 江其朋,江连强,龚杰,等.影响四川凉山地区烟草根结线虫病发生的关键因子分析[J].中国烟草学报,2021,27(6):89-98. [5] 迟元凯,叶梦迪,赵伟,等.氟吡菌酰胺对南方根结线虫的作用效果[J].植物保护学报,2019,46(6):1364-1370. [6] 范继巧,张殿斌,张治家,等.3种熏蒸剂土壤消毒对黄瓜根结线虫病的防治效果[J].山西农业科学,2020,48(9):1514-1516. [7] 刘晓宇,陈立杰,邢志富,等.4种生物源杀线剂对番茄根结线虫的田间防效[J].植物保护,2020,46(6):228-232,253. [8] 龙岗,江连强,陈树鸿,等.不同药剂对烟草根结线虫病的防控效果研究[J].植物医生,2021,34(5):23-29. [9] 祝明亮,李天飞,夏振远,等.云南省烟草根结线虫发病土壤生态因子分析[J].中国烟草学报,2003,9(4):30-34. [10] 黄阔,江其鹏,姚晓远,等.微生物菌剂对烟草根结线虫及根际微生物群落多样性的影响[J].中国烟草科学,2019,40(5):36-43. [11] 黄阔.烟草根际微生物与根结线虫发生的关系及调控作用研究[D].重庆:西南大学,2020. [12] 段玉玺.植物线虫学[M].北京:科学出版社,2011. [13] 查明迪,查友贵,张俊文,等.4种农药防治烟草根结线虫病田间药效试验[J].云南农业大学学报(自然科学),2021,36(5):783-788. [14] 杨章明,常宁涛,吴先华,等.万寿菊与厚孢轮枝菌联合对烟草根结线虫病的防治效果[J].安徽农业科学,2018,46(27):160-162. [15] 王洪山,李少龙,王宾,等.新型微生物农药厚孢轮枝菌微粒剂对温室黄瓜根结线虫的防治效果[J].中国蔬菜,2019(12):88-89. [16] 奚家勤,祝明亮,刘士清,等.云南省烟草根结线虫卵寄生菌的分离和筛选[J].烟草科技,2004,37(6):43-45. [17] 祝明亮,张克勤,李天飞,等.烟草根际厚孢轮枝菌生态效应研究[J].微生物学通报,2002,29(2):4-8. [18] 李瑞,李惠霞,谢丙炎,等.长枝木霉菌株TL16防治南方根结线虫的作用机理[J].植物保护学报,2020,47(2):384-393. [19] 张雅静,宋美燕,张怡静,等.兼防黄瓜根腐病和根结线虫病的淡紫拟青霉和哈茨木霉的筛选[J].生物技术通报,2021,37(2):40-50. [20] 王海明.防控南方根结线虫的木霉菌肥研制[D].沈阳:沈阳农业大学,2020. [21] 黎妍妍,李春黎,王林,等.生防菌使用方式对烟草青枯病的防效及根际土壤细菌群落的影响[J].中国烟草学报,2020,26(4):101-107. [22] 杜相革,曲再红.有机土壤改良剂和施用方式对番茄早疫病的防治效果[J].中国农学通报,2004,20(6):71-72. [23] MAKHUBU F N,KHOSA M C,MCGAW L J.South African Plants with Nematicidal Activity Against Root-Knot Nematodes:a Review[J].South African Journal of Botany,2021,139:183-191. [24] 赵芳,查宏波,陈旭,等.生物农药配合施用对连作烟地土传病害防效及烟叶产质量的影响[J].贵州农业科学,2019,47(2):37-40. [25] FEI D,PEIBO L,QUAN Z,et al.Toxicity Test of Different Pesticides on Tobacco Root-knot Nematodes[J].Agricultural Biotechnology,2020,9(5):86-89. [26] DHILLON N K,KAUR S,ANUPAM A,et al.Allelopathic Potential of Streptomyces Lydicus Strain WYEC 108 and Verticillium Chlamydosporium Strain IIHR VC-3 to Control Root Knot Nematode (Meloidogyne Incognita) in Tomato[J].Allelopathy Journal,2020,50 (1):107-118. [27] SHAHID M,GOWEN S R,PEMBROKE B.Evaluation of Efficacy of Pasteuria Penetrans Alone and in Combination with Verticillium Chlamydosporium Against Meloidogyne Javanica[J].Plant Protection,2020,4(3):117-124.
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