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2株烟粉虱高致病力病原棒束孢菌株的毒力测定

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任丽梅, 苏正钦, 欧达, 等. 2株烟粉虱高致病力病原棒束孢菌株的毒力测定[J]. 植物医学, 2022, (3): 86-93. doi: 10.13718/j.cnki.zwyx.2022.03.013
引用本文: 任丽梅, 苏正钦, 欧达, 等. 2株烟粉虱高致病力病原棒束孢菌株的毒力测定[J]. 植物医学, 2022, (3): 86-93. doi: 10.13718/j.cnki.zwyx.2022.03.013
shu
REN Limei, SU Zhengqin, OU Da, et al. Pathogenicity Evaluation of Two Isaria strains for Whitefly Bemisia tabaci Biological Control[J]. PLANT HEALTH AND MEDICINE, 2022, (3): 86-93. doi: 10.13718/j.cnki.zwyx.2022.03.013
Citation: REN Limei, SU Zhengqin, OU Da, et al. Pathogenicity Evaluation of Two Isaria strains for Whitefly Bemisia tabaci Biological Control[J]. PLANT HEALTH AND MEDICINE, 2022, (3): 86-93. doi: 10.13718/j.cnki.zwyx.2022.03.013
shu

2株烟粉虱高致病力病原棒束孢菌株的毒力测定

  • 1. 广西大学 农学院植物科学国家级实验教学示范中心, 南宁 530004;

  • 2. 华南农业大学 生物防治教育部工程研究中心, 广州 510642;

  • 3. 重庆师范大学 媒介昆虫重庆市重点实验室, 重庆 401331

详细信息
    作者简介:

    任丽梅,博士研究生,主要从事植物保护研究. .

  • 中图分类号: S435.72

Pathogenicity Evaluation of Two Isaria strains for Whitefly Bemisia tabaci Biological Control

  • 1. National Demonstration Center for Experimental Plant Science Education, College of Agriculture, Guangxi University, Nanning 530004, China;

  • 2. Engineering Research Center of Biological Control, Ministry of Education, Guangzhou 510642, China;

  • 3. Chongqing Key Laboratory of Vector Insects, Chongqing Normal University, Chongqing 401331, China

  • 摘要: 烟粉虱（Bemisiatabaci）是世界性分布且危害严重的蔬菜花卉害虫.由于烟粉虱的抗药性及传统化学药剂施用潜在的负面影响，利用病原微生物、天敌昆虫等开展烟粉虱的生物防治一直是国内外学者们研究的重点与热点之一.本研究在室内条件下，分析了爪哇棒束孢（Isaria javanica） GZQ-1菌株和玫烟色棒束孢（Isariafumosorosea） YMS01菌株对烟粉虱（MEAM1隐种）不同虫态的致病力.结果表明，2种棒束孢昆虫病原真菌均能侵染烟粉虱各个龄期，且随着孢子浓度的升高，烟粉虱的死亡率不断上升；在1×108孢子/mL时，2株棒束孢对烟粉虱2龄若虫的感染致死率分别达到92.68%和78.12%；此外，2株棒束孢菌株对2龄若虫的致病力回归方程分别为y=0.421x-1.665，y=0.33x-1.646，LC50值分别为9.01×103孢子/mL和9.74×104孢子/mL，LT50为3.05 d和4.56 d.综上所述，本研究所评价的2株棒束孢均对烟粉虱有较高的致病力，且爪哇棒束孢对烟粉虱致病力优于玫烟色棒束孢，可以将2株棒束孢作为烟粉虱的优良生防菌株开展更深入的应用研究.
    Abstract: Bemisia tabaci is a serious agricultural pest across the world. Entomopathogenic fungi, being an important external control factor of natural ecological regulation, can effectively reduce the number of pests through infection of insect thus showing an ideal control effect. This study investigated the pathogenicity of two Isaria strains, I. javanica GZQ-1 and I. fumosorosea YMS01, against the Middle East-Asia Minor (MEAM1) cryptic species of B. tabaci. The results showed that both strains can infect all the life stages of Bemisia tabaci and virulence was enhanced in a dose depend manner. The pathogenicity of I. javanica GZQ-1 and I. fumosorosea YMS01 (when the concentration was 1×108 conidia/mL) to the 2nd instar nymphs of B. tabaciwas 92.68% and 78.12%, respectively, theregression equations for I. javanica GZQ-1 and I. fumosorosea YMS01 pathogenicity werey=0.421 x-1.665, y=0.33 x-1.646, respectively. The median lethal concentration (LC50) of I. javanica GZQ-1 and I. fumosorosea YMS01 to the 2nd instar nymphs were 9.01× 103 and 9.74 × 104 conidia/mL, respectively, while their median lethal time (LT50) values to whitefly were 3.051 days and 4.557 days, respectively. In summary, the two Isaria strains used in current experiment can be excellent biological control agents of B. tabaci, and the pathogenic potential of I. javanica GZQ-1 was higher than I. fumosorosea YMS01. These results will improve the overall knowledge on application of entomopathogenic fungi forbiological control of B. tabaci.
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  • [1] DE BARRO P J, DRIVER F, NAUMANN I D, et al. Descriptions of Three Species of Eretmocerus haldeman(Hymenoptera:Aphelinidae) Parasitising Bemisiatabaci (Gennadius) (Hemiptera:Aleyrodidae) and Trialeurodes vaporariorum (Westwood) (Hemiptera:Aleyrodidae) in Australia Based on Morphological and Mol[J]. Australian Journal of Entomology, 2000, 39(4):259-269.
    [2] 梁宗琦. 昆虫病原真菌的研究利用[J]. 贵州农业科学, 1983, 11(5):21-28.
    [3] MEYLING N V, EILENBERG J. Ecology of the Entomopathogenic Fungi Beauveria bassiana and Metarhizium anisopliae in Temperate Agroecosystems:Potential for Conservation Biological Control[J]. Biological Control, 2007, 43(2):145-155.
    [4] 邱德文. 生物农药研究进展与未来展望[J]. 植物保护, 2013, 39(5):81-89.
    [5] 范月蕾, 赵晓勤, 陈大明, 等. 微生物杀虫剂研发现状和产业化发展态势[J]. 生物产业技术, 2016(1):54-58.
    [6] 蒲蛰龙. 应用昆虫的病原真菌防治害虫[J]. 生物防治通报, 1985, 1(1):27-31.
    [7] SCORSETTI A C, HUMBER R A, GREGORIO C, et al. New Records of Entomopathogenic Fungi Infecting Bemisia tabaci and Trialeurodes vaporariorum, Pests of Horticultural Crops, in Argentina[J]. BioControl, 2007, 53(5):787-796.
    [8] 黄勃, 王四宝, 金伟, 等. 家蚕灰僵病病原的鉴定[J]. 蚕业科学, 2008, 34(2):257-261.
    [9] ZIMMERMANN G. The Entomopathogenic Fungi Isaria farinosa (Formerly Paecilomyces farinosus) and the Isaria Fumosorosea Species Complex (Formerly Paecilomyces fumosoroseus):Biology, Ecology and Use in Biological Control[J]. Biocontrol Science and Technology, 2008, 18(9):865-901.
    [10] MAKETON M, OROSZ-COGHLAN P, JAENGARUN J. Note:Field Evaluation of Isaria fumosorosea in Controlling the Diamondback Moth (Plutella xylostella) in Chinese Kale[J]. Phytoparasitica, 2008, 36(3):260-263.
    [11] BUITENHUIS R, BROWNBRIDGE M, BROMMIT A, et al. How to Start with a Clean Crop:Biopesticide Dips Reduce Populations of Bemisia tabaci (Hemiptera:Aleyrodidae) on Greenhouse Poinsettia Propagative Cuttings[J]. Insects, 2016, 7(4):E48.
    [12] MAHERMATTER M, MAHMOUD S M. Differential Efficacies of Nomuraea rileyi and Isaria fumosorosea on some Serious Pests and the Pests'Efficient Predator Prevailing in Tomato Fields in Egypt[J]. Journal of Plant Protection Research, 2013, 53(2):103-109.
    [13] 张仙红. 玫烟色拟青霉生物学特性及感病菜青虫病理变化的研究[D]. 太谷:山西农业大学, 2005.
    [14] 吕利华, 武亚敬, 何余容, 等. 玫烟色拟青霉亚致死浓度对小菜蛾取食和成虫生殖的影响[J]. 中国生物防治, 2007, 23(4):322-327.
    [15] 黄振, 任顺祥, 吴建辉. 玫烟色拟青霉对烟粉虱种群的控制作用[J]. 华南农业大学学报, 2007, 28(2):24-28.
    [16] 景云飞, 张仙红. 昆虫病原真菌玫烟色拟青霉的研究进展[J]. 山西农业科学, 2007, 35(9):19-22.
    [17] VIDALC, LACE L A, FARGUESJ. Pathogenicity of Paecilomyces fumosoroseus(Deuteromycotina:Hyphomycetes) Against Bemisia argentifolii (Homoptera:Aleyrodidae) with a Description of a Bioassay Method[J]. Journal of Economic Entomology, 1997, 90(3):765-772.
    [18] LABANOWSKI G S, SOIKA G. Effectiveness of Microbial and Botanical Insecticides in the Control of BemisiaTabaci and Frankliniella occidentalis on Ornamental Plants[J]. EPPO Bulletin, 1999, 29(1-2):77-80.
    [19] 田晶, 梁丽, 李新凤, 等. 玫烟色棒束孢IF-1106菌株对烟粉虱的致病力[J]. 山西农业科学, 2013, 41(7):728-731.
    [20] CHEN C C, KUMAR H G A, KUMAR S, et al. Molecular Cloning, Characterization, and Expression of a Chitinase from the Entomopathogenic Fungus Paecilomyces javanicus[J]. Current Microbiology, 2007, 55(1):8-13.
    [21] 田晶, 田浩楷, 刁红亮, 等. 玫烟色棒束孢对烟粉虱致病的时间-剂量-死亡率模型分析[J]. 河南农业科学, 2017, 46(1):70-75.
    [22] 张仙红, 李文英, 贺运春. 影响虫生真菌玫烟色拟青霉孢子萌发的因素探析[J]. 中国农学通报, 2007, 23(7):478-481.
    [23] 田晶. 玫烟色棒束孢生物学特性及对烟粉虱致病作用研究[D]. 太谷:山西农业大学, 2014.
    [24] 王兴民, 罗莹, 龙秀珍, 等. 紫外线诱变对玫烟色棒束孢生物学特性的影响[J]. 南方农业学报, 2015, 46(7):1213-1217.
    [25] 田晶, 刁红亮, 马瑞燕. 玫烟色棒束孢侵染对烟粉虱成虫体内不同酶活的影响[J]. 山西农业科学, 2016, 44(7):1007-1010.
    [26] 张维, 彭国雄, 夏玉先. 昆虫病原真菌防控草地贪夜蛾的现状、问题与展望[J]. 中国生物防治学报, 2019, 35(5):674-681.
    [27] SHIMAZU M, TAKATSUKA J. Isaria javanica (Anamorphic Cordycipitaceae) Isolated from Gypsy Moth Larvae, Lymantria dispar(Lepidoptera:Lymantriidae), in Japan[J]. Applied Entomology and Zoology, 2010, 45(3):497-504.
    [28] AYALA-ZERMEÑO M A, GALLOU A, BERLANGA-PADILLA A M, et al. Characterisation of Entomopathogenic Fungi Used in the Biological Control Programme of Diaphorina citri in Mexico[J]. Biocontrol Science and Technology, 2015, 25(10):1192-1207.
    [29] KIM J J, XIEL, HAN J H, et al. Influence of Additives on the Yield and Pathogenicity of Conidia Produced by Solid State Cultivation of an Isaria javanica Isolate[J]. Mycobiology, 2014, 42(4):346-352.
    [30] LI X, HAN J H, KIM J J, et al. Effects of Culture Conditions on Conidial Production of the Sweet Potato Whitefly Pathogenic Fungus Isaria javanica[J]. Mycoscience, 2016, 57(1):64-70.
    [31] HERRERO N. Identification and Sequence Determination of a New ChrysovirusInfecting the Entomopathogenic Fungus Isaria javanica[J]. Archives of Virology, 2017, 162(4):1113-1117.
    [32] NGUYEN T T, TRUONG X S, THAI T N L. Pathogenicity Assessment of Isaria javanica (Frider&Bally) Samson&Hywel-JonesIsolates Against Spodoptera litura Fabr[J]. Biological Forum, 2017, 9(1):189-193.
    [33] KANG B R, HAN J H, KIM J J, et al. Dual Biocontrol Potential of the Entomopathogenic Fungus, Isaria javanica, for both Aphids and Plant Fungal Pathogens[J]. Mycobiology, 2018, 46(4):440-447.
    [34] CHENG X, GAO Y Y, YANG C L. Potential of Entomopathogenic Fungus Isaria javanica for Controlling the Potato Tuberworm Phthorimaea operculella(Zeller) (Lepidoptera:Gelechiidae)[J]. Israel Journal of Entomology, 2018, 48(2):197-208.
    [35] PARK S E, KIM J C, LEE S J, et al. Solid Cultures of Thrips-Pathogenic Fungi Isaria javanica Strains for Enhanced Conidial Productivity and Thermotolerance[J]. Journal of Asia-Pacific Entomology, 2018, 21(4):1102-1109.
    [36] 展茂魁, 何玲敏, 陈名, 等. 防治假眼小绿叶蝉的虫生真菌高毒菌株的筛选及田间防治效果[J]. 中国生物防治学报, 2012, 28(1):41-46.
    [37] 陈名, 张大敏, 彭凡, 等. 用于防治假眼小绿叶蝉的爪哇棒束孢可湿性粉剂的研制[J]. 中国生物防治学报, 2014, 30(1):51-57.
    [38] 邓嘉茹, 孙龙华, 毕可可, 等. 埃及吹绵蚧昆虫病原真菌的筛选、鉴定及其致病性测定[J]. 植物保护学报, 2020, 47(1):53-64.
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  • 收稿日期:  2022-06-01

2株烟粉虱高致病力病原棒束孢菌株的毒力测定

    作者简介: 任丽梅,博士研究生,主要从事植物保护研究.
  • 1. 广西大学 农学院植物科学国家级实验教学示范中心, 南宁 530004;
  • 2. 华南农业大学 生物防治教育部工程研究中心, 广州 510642;
  • 3. 重庆师范大学 媒介昆虫重庆市重点实验室, 重庆 401331
  • 收稿日期:  2022-06-01
基金项目: 

摘要: 烟粉虱（Bemisiatabaci）是世界性分布且危害严重的蔬菜花卉害虫.由于烟粉虱的抗药性及传统化学药剂施用潜在的负面影响，利用病原微生物、天敌昆虫等开展烟粉虱的生物防治一直是国内外学者们研究的重点与热点之一.本研究在室内条件下，分析了爪哇棒束孢（Isaria javanica） GZQ-1菌株和玫烟色棒束孢（Isariafumosorosea） YMS01菌株对烟粉虱（MEAM1隐种）不同虫态的致病力.结果表明，2种棒束孢昆虫病原真菌均能侵染烟粉虱各个龄期，且随着孢子浓度的升高，烟粉虱的死亡率不断上升；在1×108孢子/mL时，2株棒束孢对烟粉虱2龄若虫的感染致死率分别达到92.68%和78.12%；此外，2株棒束孢菌株对2龄若虫的致病力回归方程分别为y=0.421x-1.665，y=0.33x-1.646，LC50值分别为9.01×103孢子/mL和9.74×104孢子/mL，LT50为3.05 d和4.56 d.综上所述，本研究所评价的2株棒束孢均对烟粉虱有较高的致病力，且爪哇棒束孢对烟粉虱致病力优于玫烟色棒束孢，可以将2株棒束孢作为烟粉虱的优良生防菌株开展更深入的应用研究.

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