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苹果炭疽叶枯病病原菌分离鉴定及防治药剂的室内测定

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吕昭龙, 李继平, 郑果, 等. 苹果炭疽叶枯病病原菌分离鉴定及防治药剂的室内测定[J]. 植物医学, 2023, (1): 34-43. doi: 10.13718/j.cnki.zwyx.2023.01.005
引用本文: 吕昭龙, 李继平, 郑果, 等. 苹果炭疽叶枯病病原菌分离鉴定及防治药剂的室内测定[J]. 植物医学, 2023, (1): 34-43. doi: 10.13718/j.cnki.zwyx.2023.01.005
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LÜ Zhao-long, LI Ji-ping, ZHENG Guo, et al. Isolation and Identification of Pathogen of Apple Glomerella Leaf Spot and Laboratory Determination of Control Agents[J]. PLANT HEALTH AND MEDICINE, 2023, (1): 34-43. doi: 10.13718/j.cnki.zwyx.2023.01.005
Citation: LÜ Zhao-long, LI Ji-ping, ZHENG Guo, et al. Isolation and Identification of Pathogen of Apple Glomerella Leaf Spot and Laboratory Determination of Control Agents[J]. PLANT HEALTH AND MEDICINE, 2023, (1): 34-43. doi: 10.13718/j.cnki.zwyx.2023.01.005
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苹果炭疽叶枯病病原菌分离鉴定及防治药剂的室内测定

  • 1. 甘肃省农业科学院 植物保护研究所, 兰州 730070;

  • 2. 甘肃农业大学 植物保护学院, 兰州 730070

详细信息
    作者简介:

    吕昭龙,硕士研究生,主要从事植物病害及其综合防治研究. .

  • 中图分类号: S432

Isolation and Identification of Pathogen of Apple Glomerella Leaf Spot and Laboratory Determination of Control Agents

  • 1. Institute of Plant Protection, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China;

  • 2. College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, China

  • 摘要: 本研究对采自甘肃省礼县的苹果炭疽叶枯病病原菌种类进行了鉴定,并开展了防治药剂的室内筛选.通过组织分离法将病原菌分离纯化后,采用科赫氏法则进行致病性验证,结合形态学特征和内转录间隔区基因(Internal Transcribed Spacer,ITS)、 β-微管蛋白基因(β-Tubulin,TUB2)和肌动蛋白基因(Actin,ACT)多基因的系统发育分析对病原菌进行鉴定;并利用菌丝生长速率法测定6种化学杀菌剂对病原菌的抑制效果.结果表明,分离菌株为隐秘刺盘孢(Colletotrichum aenigma),可同时侵染叶片和果实,为当地苹果炭疽叶枯病致病菌.室内毒力测定结果表明,杀菌剂丙森锌和嘧菌环胺对菌丝的生长抑制效果较好,其EC50值分别为0.698 0 mg/L和1.232 6 mg/L;抑霉唑和异菌脲抑菌效果次之,EC50值分别为3.688 9 mg/L和2.684 0 mg/L;氰霜唑和氟啶胺抑菌效果较差,EC50值分别为58.740 9 mg/L和33.647 7 mg/L.研究结果为苹果炭疽叶枯病的有效防治提供了参考依据.
    Abstract: The purpose of this study was to determine the species of apple glomerella leaf spot in Li City, Gansu Province and to screen the control fungicides. In this study, the leaves suspected of being infected with apple glomerella leaf spot were used for isolation of the pathogen by tissue isolation method. The pathogenicity was verified by Koch's rule. The phylogenetic analysis was performed with Internal Transcribed Spacer(ITS), the β-tubulin gene(TUB2), and the Actin gene(ACT) to identify the pathogen in combining with the morphological characteristics. The inhibitory effect of 6 kinds of chemical fungicides on isolates was determined by mycelial growth rate method. The results showed that the isolate was Colletotrichumaenigma.Its pathogenicity showed that it could infect not only leaves but also fruits. The results of laboratory toxicity test showed that the Cyprodinil and Propineb had better inhibitory effect on the growth of mycelia with EC50 value of 0.698 0 mg/L and 1.232 6 mg/L, respectively. The effects of Imazalil and Iprodione were less with EC50 value of 3.688 9 mg/L and 2.684 0 mg/L, respectively. The effects of Cyazofamid and Fluazinam were the least with EC50 value of 58.940 9 mg/L and 33.647 7 mg/L, respectively. This study provides a reference for the prevention and treatment of apple glomerella leaf spot.
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  • 收稿日期:  2022-11-20

苹果炭疽叶枯病病原菌分离鉴定及防治药剂的室内测定

    作者简介: 吕昭龙,硕士研究生,主要从事植物病害及其综合防治研究.
  • 1. 甘肃省农业科学院 植物保护研究所, 兰州 730070;
  • 2. 甘肃农业大学 植物保护学院, 兰州 730070
  • 收稿日期:  2022-11-20
基金项目: 

摘要: 本研究对采自甘肃省礼县的苹果炭疽叶枯病病原菌种类进行了鉴定,并开展了防治药剂的室内筛选.通过组织分离法将病原菌分离纯化后,采用科赫氏法则进行致病性验证,结合形态学特征和内转录间隔区基因(Internal Transcribed Spacer,ITS)、 β-微管蛋白基因(β-Tubulin,TUB2)和肌动蛋白基因(Actin,ACT)多基因的系统发育分析对病原菌进行鉴定;并利用菌丝生长速率法测定6种化学杀菌剂对病原菌的抑制效果.结果表明,分离菌株为隐秘刺盘孢(Colletotrichum aenigma),可同时侵染叶片和果实,为当地苹果炭疽叶枯病致病菌.室内毒力测定结果表明,杀菌剂丙森锌和嘧菌环胺对菌丝的生长抑制效果较好,其EC50值分别为0.698 0 mg/L和1.232 6 mg/L;抑霉唑和异菌脲抑菌效果次之,EC50值分别为3.688 9 mg/L和2.684 0 mg/L;氰霜唑和氟啶胺抑菌效果较差,EC50值分别为58.740 9 mg/L和33.647 7 mg/L.研究结果为苹果炭疽叶枯病的有效防治提供了参考依据.

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