高效氯氟氰菊酯亚致死浓度对番石榴实蝇代谢酶的影响
On Effects of Sublethal Doses on Metabolic Enzymes of Lambda-Cyhalothrin to Bactrocera Correcta (Bezzi)
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摘要: 为了解高效氯氟氰菊酯亚致死浓度对番石榴实蝇 Bactrocera correcta(Bezzi)代谢酶的影响,采用药膜法测定了高效氯氟氰菊酯对番石榴实蝇不同种群的毒力及代谢酶活性.结果表明:经药剂选育15代后,种群抗性倍数为11.50,达到中等抗性.在LC10,LC25和LC50处理下,敏感种群的羧酸酯酶(CarE)和谷胱甘肽S 转移酶(GST)显著提升(p<0.05),而抗性种群的CarE和GST无统计学意义.不同种群的过氧化氢酶(CA T)和过氧化物酶(POD)活性无统计学意义.表明 CarE和GST活性的提升与敏感种群的抗性发展有关.Abstract: In order toeffects of sublethal doses on metabolic enzymes of lambda‐cyhalothrin to Bactrocera correcta (Bezzi) ,the toxicity and metabolic enzymes of lambda‐cyhalothrin todifferent populationsof B . correctahave beendetectedinresidual film method .The results show that the population resistance ratiois 11 .50 after the reagent selected 15 generations ,it reaches medium resistance level .Under LC10 ,LC25 and LC50 of lambda‐cyhalothrin ,activity of CarE and GSTare significantly increased in susceptible population (p<0 .05) ,but activity of CarE and GSTareno significant in resistant population .Activity of CAT and POD areno significant in different populations .It indicated thatCarE and GST activity promotion associat‐ed with the resistance development ofsusceptible population .
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