云南栘［木衣］叶提取物片段抗氧化活性及成分分析

刘刚,吴京,朱明君,张晓喻,张宏

doi:10.13718/j.cnki.xsxb.2014.09.014

西南师范大学学报(自然科学版)

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云南栘［木衣］叶提取物片段抗氧化活性及成分分析

刘刚,吴京,朱明君,张晓喻,张宏. 云南栘［木衣］叶提取物片段抗氧化活性及成分分析[J]. 西南师范大学学报(自然科学版), 2014, 39(9). doi: 10.13718/j.cnki.xsxb.2014.09.014

引用本文:

LIU Gang,WU Jing,ZHU Ming-jun,ZHANG Xiao-yu,ZHANG Hong. On Evaluation of Antioxidant Activities and Screening of Potential Antioxidant Compounds from Docynia delavayi (Franch .) Schneid .Leaves[J]. Journal of Southwest China Normal University(Natural Science Edition), 2014, 39(9). doi: 10.13718/j.cnki.xsxb.2014.09.014

Citation:

LIU Gang,WU Jing,ZHU Ming-jun,ZHANG Xiao-yu,ZHANG Hong. On Evaluation of Antioxidant Activities and Screening of Potential Antioxidant Compounds from Docynia delavayi (Franch .) Schneid .Leaves[J]. Journal of Southwest China Normal University(Natural Science Edition), 2014, 39(9). doi: 10.13718/j.cnki.xsxb.2014.09.014

云南栘［木衣］叶提取物片段抗氧化活性及成分分析

刘刚,吴京,朱明君,张晓喻,张宏

四川师范大学生命科学学院,成都610101; 四川师范大学植物资源应用与开发研究所,成都610101 四川师范大学生命科学学院,成都,610101

On Evaluation of Antioxidant Activities and Screening of Potential Antioxidant Compounds from Docynia delavayi (Franch .) Schneid .Leaves

LIU Gang,WU Jing,ZHU Ming-jun,ZHANG Xiao-yu,ZHANG Hong

摘要: 目的：评价云南栘[木衣]叶萃取物的抗氧化活性，为云南栘[木衣]抗氧化功能食品、营养补充剂等的开发提供理论依据．方法：用系统溶剂萃取法将云南栘[木衣]叶的萃取物再分成石油醚萃取物、乙酸乙酯萃取物、正丁醇萃取物和水相4部分．用DPPH ·法和总还原力法评价云南栘[木衣]叶4个不同萃取物的抗氧化活性．用DPPH ·-HPLC-PDA和总还原力-HPLC-PDA柱前衍生法对云南栘[木衣]叶中的抗氧化成分进行了筛选．结果：云南栘[木衣]叶提取物不同极性分离物都具有良好的抗氧化活性，其中乙酸乙酯萃取物的萃取得率高达67.87％，DPPH ·半清除体积质量分数为0.079 m g/m L ，总还原力的芦丁质量分数为（0.19970±0.00031）g/g ，是云南栘[木衣]叶抗氧化活性的主要来源．云南栘[木衣]叶提取物中主要有9个抗氧化成分，主要集中于乙酸乙酯萃取物，其中4个为黄酮类化合物．结论：云南栘[木衣]叶是良好的天然抗氧化剂来源，具有抗氧化营养补充剂、功能食品的开发潜力，抗氧化成分筛选结果为云南栘[木衣]抗氧化功能的开发提供了方向．
- 云南栘木衣 /
- 抗氧化活性 /
- 成分分析
Abstract: The antioxidant activity of Docynia delavayiSchneid .leaf has been estimated in this paper .It provides theoretical basis for developing Docynia delavayiantioxidant nutritional supplement . Docynia delavayiSchneid .leaf extract has been separated by different polarity solvents :petroleum ether ,ethylace-tate ,n-butanol and water via systematic solvent extraction .Antioxidant activities of different polarity frac-tions have been evaluated by DPPH · assay and total reducing power assay .Its potential antioxidant com-pounds have also been screened by DPPH ·-HPLC-PDA assay and total reducing power-HPLC-PDA as-say .All polarity fractions of Docynia delavayiSchneid .leaf extract have exhibited high antioxidant activi-ty .The yield of ethylacetate fraction reached up to 67 .87% .The DPPH · SC50 value and total reducing power of ethylacetate fraction are 0 .079 mg/mL and (0 .19970 ± 0 .00031) g/g ,respectively .The antioxi-dant activity of Docynia delavayiSchneid .leaf mainly comes from ethylacetate fraction .9 antioxidants mainly concentrating in ethylacetate fraction are screened and 4 of them are flavones .It is concluded that Docynia delavayiSchneid .leaf is a good source of natural antioxidants and the result of antioxidants screening could be used to guide Docynia delavayiantioxidant nutritional supplement development .
- Docynia delavayi(Franch .) Schneid .，antioxidant activity，screening /

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云南栘［木衣］叶提取物片段抗氧化活性及成分分析

刘刚,吴京,朱明君,张晓喻,张宏

四川师范大学生命科学学院,成都610101; 四川师范大学植物资源应用与开发研究所,成都610101 四川师范大学生命科学学院,成都,610101

关键词:

摘要: 目的：评价云南栘[木衣]叶萃取物的抗氧化活性，为云南栘[木衣]抗氧化功能食品、营养补充剂等的开发提供理论依据．方法：用系统溶剂萃取法将云南栘[木衣]叶的萃取物再分成石油醚萃取物、乙酸乙酯萃取物、正丁醇萃取物和水相4部分．用DPPH ·法和总还原力法评价云南栘[木衣]叶4个不同萃取物的抗氧化活性．用DPPH ·-HPLC-PDA和总还原力-HPLC-PDA柱前衍生法对云南栘[木衣]叶中的抗氧化成分进行了筛选．结果：云南栘[木衣]叶提取物不同极性分离物都具有良好的抗氧化活性，其中乙酸乙酯萃取物的萃取得率高达67.87％，DPPH ·半清除体积质量分数为0.079 m g/m L ，总还原力的芦丁质量分数为（0.19970±0.00031）g/g ，是云南栘[木衣]叶抗氧化活性的主要来源．云南栘[木衣]叶提取物中主要有9个抗氧化成分，主要集中于乙酸乙酯萃取物，其中4个为黄酮类化合物．结论：云南栘[木衣]叶是良好的天然抗氧化剂来源，具有抗氧化营养补充剂、功能食品的开发潜力，抗氧化成分筛选结果为云南栘[木衣]抗氧化功能的开发提供了方向．

English Abstract

On Evaluation of Antioxidant Activities and Screening of Potential Antioxidant Compounds from Docynia delavayi (Franch .) Schneid .Leaves

四川师范大学生命科学学院,成都610101; 四川师范大学植物资源应用与开发研究所,成都610101 四川师范大学生命科学学院,成都,610101

Keywords:

Abstract: The antioxidant activity of Docynia delavayiSchneid .leaf has been estimated in this paper .It provides theoretical basis for developing Docynia delavayiantioxidant nutritional supplement . Docynia delavayiSchneid .leaf extract has been separated by different polarity solvents :petroleum ether ,ethylace-tate ,n-butanol and water via systematic solvent extraction .Antioxidant activities of different polarity frac-tions have been evaluated by DPPH · assay and total reducing power assay .Its potential antioxidant com-pounds have also been screened by DPPH ·-HPLC-PDA assay and total reducing power-HPLC-PDA as-say .All polarity fractions of Docynia delavayiSchneid .leaf extract have exhibited high antioxidant activi-ty .The yield of ethylacetate fraction reached up to 67 .87% .The DPPH · SC50 value and total reducing power of ethylacetate fraction are 0 .079 mg/mL and (0 .19970 ± 0 .00031) g/g ,respectively .The antioxi-dant activity of Docynia delavayiSchneid .leaf mainly comes from ethylacetate fraction .9 antioxidants mainly concentrating in ethylacetate fraction are screened and 4 of them are flavones .It is concluded that Docynia delavayiSchneid .leaf is a good source of natural antioxidants and the result of antioxidants screening could be used to guide Docynia delavayiantioxidant nutritional supplement development .