中心组合实验设计响应面法优化α淀粉酶抑制剂筛选条件
On Optimization Screening Conditions of α-Amylase Inhibitor by Central Composite Design Coupled with Response Surface Methodology
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摘要: 采用中心组合设计（CCD）响应面法（RSM ）优化α淀粉酶抑制剂筛选条件。在单因素试验基础上，对影响α淀粉酶抑制剂活性的4个因素即淀粉溶液浓度、保温温度、DNS用量、沸水时间进行优化，根据回归方程最终确定α淀粉酶溶液质量浓度1 mg／mL ，淀粉溶液质量浓度17 mg／mL ，保温温度46℃，保温时间15 min ，DNS用量2 mL ，沸水时间6 min 。经试验验证结果稳定可靠，可用于α淀粉酶抑制剂筛选。Abstract: Central Composite Design (CCD) coupled with Response Surface Method (RSM ) has been per‐formed to optimize α‐amylase inhibitor screening conditions .On the basis of single factor experiments , four factors includes starch solution concentration ,heat preservation temperature ,dosage of DNS ,boiling time that affect α‐amylase activity have been optimized .Results show as follow s :α‐amylase concentration 1mg/mL ,starch solution concentration 17 mg/mL ,preservation temperature 46 ℃ ,holding time 15min , dosage of DNS 2 mL ,boiling time 6 min .The result is stable and reliable and can be used for α‐amylase in‐hibitor screening .
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