摘要:
建立HPLC法同时测定仙桃草中芦丁、木犀草苷、木犀草素3种黄酮含量的方法.采用VenusilMPC18(2)色谱柱(4.6mm×250mm,5μm),以乙腈-0.2%磷酸溶液为流动相,梯度洗脱,流速1.0mL/min,检测波长350nm,柱温为35℃.方法学验证结果表明,3种黄酮类化合物的分离度良好,且在各自质量浓度范围内线性关系良好,r≥0.9991,平均加样回收率(n=6)在96.76%~98.03%,RSD为1.62%~1.93%.3种黄酮类化合物在不同批次仙桃草中的含量存在较大的差异,仙桃草中3种黄酮总量在373.458~673.413μg/g之间.由此表明,不同生产批次的仙桃草药材,其内在品质存在较大差异,本方法可用于仙桃草药材中多种黄酮类成分同步测定,同时可以为仙桃草的质量控制提供科学依据.
Abstract:
An HPLC procedure is established to simultaneously determine the contents of three flavonoids (rutoside, galuteolin and luteolin) in purslane speedwell (Veronica peregrina L).Using the Venusil MP C18(2) column (4.6mm×250mm, 5 μm) and acetonitrile-0.2% phosphoric acid as the mobile phase, gradient elution was performed at a flow rate of 1.0 mL/min, a column temperature of 35℃, and a detection wavelength of 350nm.The results of methodological validation suggested that all the three flavonoids presented good resolution and good linearity within their respective concentration range (by mass), r being ≥0.9991, the mean recovery (n=6) being 96.76%-98.03% and RSD being 1.62%-1.93%.The contents of the three flavonoids varied significantly in different batches of purslane speedwell, and their total content varied between 373.458 and 673.413 μg/g, thus indicating a substantial variability of quality of different batches of purslane speedwell.In conclusion, this method is applicable to simultaneous determination of various flavonoids in purslane speedwell, which could provide evidences for the quality control of purslane speedwell.
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