柑橘皮渣降解菌的筛选及产酶条件优化
摘要: 柑橘加工产生大量皮渣,严重污染环境,亟须处理.本试验从堆肥中分离得到几株耐高温细菌,以CMC和果胶为唯一碳源,再经刚果红染色法初筛得到菌株L207和L702;以枯草芽孢杆菌 Bacillus subtilis L520为对照,其纤维素酶活性和果胶酶活性显著大于对照菌株( p=0.05),表明L207和L702具有很好的降解柑橘皮渣能力.对菌株L207和L702产酶条件初步研究表明:菌株L207在接种量为4%(V/V )、pH6于40℃、培养60 h时纤维素酶活性最高,在40℃,pH6、培养48 h时果胶酶活性最高;菌株 L702在接种量为6%(V/V ),pH6于40℃,培养72 h时纤维素酶活性最高;在40℃,pH6,培养36 h果胶酶活性最高.菌株L207和L702在柑橘皮渣降解处理过程具有潜在的开发价值.
Screening of Cit rus Peel-Degrading Bacteria and the Optimization of Enzyme-Production Conditions
Abstract: A large amount of Citrus peels are generated with citrus processing ,which leads to severe envi‐ronmental pollution and ,therefore ,is an urgent problem to be solved .In this study ,several strains of high‐temperature‐resistant bacteria were isolated from compost and cultured with CMC and pectin as car‐bon sources .After a primary screening with Congo red staining ,two strains ,L207 and L702 ,were select‐ed .Cellulase activity and pectinase activity of these two strains were remarkably higher than those of the control ,Bacillus subtilis strain L5 (p=0.05) ,indicating that they were endowed with an excellent capa‐bility of citrus peel degradation .In our preliminary study for their fermentation conditions ,the optimum conditions of L207 for enzyme production were:initial inoculum concentration 4% (V/V) ,fermentation temperature 40 ℃ ,pH value 6.0 ,and fermentation time 48 h (with the best resulting pectinase activity) or 60 h (with the best resulting cellulase activity);the optimum conditions of L702 for enzyme production were:initial inoculum concentration 6% (V/V) ,fermentation temperature 40 ℃ ,pH value 6.0 ,and fer‐mentation time 36 h (with the best resulting pectinase activity) or 72 h (with the best resulting cellulase activity) .Hence these two strains are of potential development value in the processing of citrus peels .