Bt杀虫蛋白基因在转基因油菜中的动态表达与其抗虫性研究
DYNAMICS OF EXPRESSION OF Bt INSECTICIDAL PROTEIN GENE IN THE TRANSGENIC RAPESEED AND ITS INSECT- RESISTANCE ACTIVITY
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摘要: 采用ELISA方法检测Bt杀虫基因在转基因油菜植株中的表达。试验结果表明：在第3至第9叶期Bt杀虫蛋白基因在转基因油莱植株中稳定、高效地表达，Bt杀虫蛋白的表达量为170ng/25mg～260ng25/mg鲜叶，占植物可溶性蛋白的0.067，～0.105，，其抗虫效果高达42.5，～80.0，。但从第11叶期后Bt杀虫蛋白表达量显著地降低，只有7ng/25mg～50ng/25mg鲜叶，占植物可溶性蛋白的0.003，～0.02，。Abstract: ELISA was adopted to analyze the expression of the insecticidal protein gene Bt in the transgenic rapeseed plants. The results showed that the expression of the protein gene was stable and highly efficient at the 3～9-leaf stage of the plants, with an expressing quantity of 170～260ng per25 mg fresh leaf,accounting for 0.067％～0.105％ of the total soluble proteins of the plant and with an insecticidal activity of ashigh as 42.5%～80.0％ .From the 11-leaf stage onward,the expressing quantity of Bt dropped sharply, being 7～50 ng per 25 mg fiesh leaf, accounting for 0.003％～0.02％ of the total soluble proteins of the plants.
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