球孢白僵菌的生物学特性及对小麦蚜虫的毒力
BIOLOGICAL CHARACTERISTICS OF THREE ISOLATES OF BEAUVERIA BASSIANA AND THEIR VIRULANCE AGAINST WHEAT APHIDS
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摘要: 3株球孢白僵茵(Beauveria bassiana)Bb 02，Bb 03和Bb 07在不同温度，pH值和培养基上培养，结果表明，三者的最适生长温度在25℃～30℃间；Bb 02和Bb 07在pH4.0～6.0生长良好；适量的蛋白胨或酵母浸膏可促进菌株的生长，但对葡萄糖和麦芽糖的利用能力没有明显差异，三者在PPDA，SDAY和SMAY上生长良好。生物测定表明，3茵株对小麦蚜虫均具有较强的毒力，但各菌株的最适剂量略有差异，其中Bb 02以106mL-1较佳，Bb 03以107mL-1最好，Bb07则介于107mL-1～108mL-1之间。
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关键词:
- 球孢白僵菌,蚜虫,毒力
Abstract: Three isolates of Beauveria bassiana, Bb 02, Bb 03 and Bb 07, were cultured at different temperature and pH and on different media. The optimum temperature for their growth ranged from 25℃ to 30℃ and Bb 02 and Bb 07 grew well at pH 4.0 ～ 6.0. The addition of peptone and yeast extract stimulated their growth,while glucose and maltose had little promoting effect. All the three isolmes grew well on the media PPDA,SDAY and SMAY. Bioassay showed that they were strongly virulent against whest aphids,though their optimum dosage varied a little. The opimum concentration was 106/mL for Bb 02,107/mL for Bb 03 and 107 ～ 108/mL for Bb 07. -
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