非洲菊花托培养和植株再生
FLORAL RECEPTACLE CULTURE IN VITRO AND PLANTLET REGENERATION IN GERBERA JAMESONII BOLUS
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摘要: 建立了以非洲菊幼小花托为外植体的离体快繁技术,繁殖系数可达5倍/20天~7倍/20天左右。诱导培养基以B5+l0 mg/L BA+0.1 mg/L IAA最佳,继代增殖以MS+1 mg/L BA+0.1mg/L LAA为宜,生根培养以1/2MS+0.01mg/L IBA效果最好。细胞学和形态学观察表明再生植株的形态和染色体无异常现象,遗传性稳定好。
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关键词:
- 非洲菊,组织培养,植株再生
Abstract: The floral receptacles of 4 cultivars of Gerbera jamesonii Bolux were used as explants for rapid micropropagation. A satisfactory procedure was established with a multiplication rate of 5 times/20d~7d. The optimum medium was B5+10mg/L BA+0.1 mg/L IAA for sahoot induction,MS+1 mg/L BA+0.1 mg/L IAA for subculture and 1/2MS+0.01mg/L IAA for rooting. The regenerated plantlete proved to be genetically stable with no abnormality when examined cytogically and morphologically. -
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