摘要:
在酸性条件下,氢溴酸加兰他敏被质子化后与AuCl4-形成离子缔合物被二氯甲烷所萃取,当缔合物被带入含鲁米诺的氯化十六烷基三甲基铵(Cetyltrimethylammonium Chlorine,CTAC)逆胶束纳米微反应器中时,离解出来的AuCl4-与鲁米诺产生化学发光.发光强度与氢溴酸加兰他敏的含量呈线性关系,从而可间接测定氢溴酸加兰他敏的含量.在优化的试验条件下,线性范围为0.001～15 μg/mL,检出限(3σ)为0.05 ng/mL,对浓度为1.0 μg/mL的氢溴酸加兰他敏进行11次平行测定,相对标准偏差(RSD)为2.24，.该法已成功用于片剂、针剂和生物体液中氢溴酸加兰他敏的测定.
Abstract:
Under the acidic condition, galantamine hydrobromide was protonated and formed an ion-complex with the negative ion AuCl4-, and the ion-complex was extracted by dichloromethane. When the ioncomplex entered a reversed micellar nanometer microreactor of cetyltrimethylammonium chlorine containing luminol, the dissociated AuCl4- reacted with luminol and produced an analytical chemiluminescnece signal. Under the optimum conditions, the CL response was linear to galantamine hydrobromide concentration ranging from 0. 001 to 15 g/mL and the limit of detection was 0.05 ng/mL with a relative standard deviation (n=11) of 2.24% for 1.0 g/mL galantamine hydrobromide. The method has been successfully applied to the determination of the studied drug in tablets, injections and biological fluids.
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