摘要:
目的:研究高血压候选基因β3肾上腺素能受体基因β3-Adrenergic receptor(ADRB3)在日本人群的分布,并探讨ADRB3基因Trp64Arg多态性与原发性高血压病(essential hypertension,EH)的关系.方法:采用流行病学调查方法分析肥胖指数、血浆胆固醇和甘油三酯等临床指标,同时调查受检者吸烟和饮酒等生活习惯;Taqman-PCR法分析ADRB3基因Trp64Arg多态性在大样本日本人群(样本总数2 714人,其中HT组1 328例,非高血压组(NT)1 386例)的分布及其与临床指标间的相关性.结果:除总胆固醇外,其余指标如年龄、性别、肥胖指数、舒张期血压、收缩期血压、HDL-脂蛋白和甘油三酯等在Trp/Trp、Trp/Arg和Arg/Arg这3类基因型携带者中均无显著性差异;卡方检验结果显示ADRB3的Trp64Arg基因型(p=0.425)与等位基因频率(p=0.501)在HT与NT之间没有显著统计学意义;ADRB3的Trp64Arg基因多态性与高血压病Logistic回归模型分析结果显示,卡方值(wald)为0.356;比值比为0.884;95，的可信区间为0.589～1.326,p值为0.551.结论:按照p＜0.05统计学标准,ADRB3的Trp64Arg多态性可能与日本人群EH无关.
Abstract:
To investigate the correlation between Trp64Arg polymorphism in β3-adrenergic gene and essential hypertension (EH) in large scale Japanese, a case-control study consisting of 1 328 essential hypertensive subjects (HT) and 1 386 normotensive controls (NT) was performed. All subjects were urban residents randomly selected from the Ehime Prefecture of Japan. Body mass index (BMI) was recorded in the epidemiological investigation process, some biochemical indicators (HDL, LDL, triglyceride and cholesterol) were measured from the blood plasma of the subjects. Meanwhile, we investigated some life-style factors of the subjects, including smoking and alcohol use. The ADRB3 Trp64Arg polymorphisms were determined in DNA extracted from peripheral blood leukocytes by Taqman-polymerase chain reaction (Taqman-PCR). Except total cholesterol, the other indicators studied (age, sex, BMI, SBP, DBP and HDL)did not differ significantly among the Trp/Trp, Trp/Arg and Arg/Arg genotype carriers. Chi-square anal ysis showed no statistically significant differences in either genotype distribution (p=0. 425) or allele frequencies (p=0. 501) between hypertensive and normotensive subjects. The logistic regression model results demonstrated that there were no association between ADRB3 gene Trp64Arg polymorphisms and hypertension subjects, and Wald=0. 356, Odd=0.884, 95%CI was 0.589-1.326, and p=0.551. Therefore, the present study suggests that the ADRB3 gene Trp64Arg polymorphisms might not be significantly associated with hypertension in homogeneous Japanese.
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