芥菜LFY同源基因克隆及序列分析

西南大学学报（自然科学版）

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芥菜LFY同源基因克隆及序列分析

张敏,汤青林,宋明,王志敏,刘婷. 芥菜LFY同源基因克隆及序列分析[J]. 西南大学学报（自然科学版）, 2009, 31(4).

引用本文:

张敏,汤青林,宋明,王志敏,刘婷. 芥菜LFY同源基因克隆及序列分析[J]. 西南大学学报（自然科学版）, 2009, 31(4).

Cloning and Analysis of LFY- like Gene from Brassica juncea Coss.[J]. Journal of Southwest University Natural Science Edition, 2009, 31(4).

Citation:

Cloning and Analysis of LFY- like Gene from Brassica juncea Coss.[J]. Journal of Southwest University Natural Science Edition, 2009, 31(4).

摘要: 在提取芥菜基因组DNA的基础上,克隆了芥菜中的LEAFY(LFY)同源基因,该基因长为1 307 bp.经过对该基因片段的核苷酸序列分析表明,它和花椰菜的BOFH基因在结构上有高度的保守性,同源率高达93，,与拟南芥中的LFY基因同源性达87，.
- 芥菜,LFY同源基因,克隆
Abstract: The homologous DNA fragment of L FY was cloned from mustard genomic DNA that had been isolated from Brassica juncea Coss. Its length was 1307kb. Analysis of the nucleotide sequence indicated that homology was up to 93% between it and BOFH of cauliflower, and up to 87% between it and LFY of Arabidopsis thaliana, which implies that they are highly conserved on their structure.