RP-HPLC同时测定口服液中的咖啡酸和阿魏酸
Simultaneous Determination of Caffeic Acid and Ferulic Acid in Oral Liquid by RP-HPLC
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摘要: 采用高效液相色谱法同时测定了口服液中的咖啡酸和阿魏酸含量,用Shim-pack VP-ODS柱(150 mm×4.6 mm,0.3μm),流动相:乙腈-3，的冰醋酸水溶液(体积比为11∶89),流速:1.0 mL/min,检测波长:321 nm.咖啡酸和阿魏酸的浓度与峰面积呈良好的线性关系,其线性范围分别为0.126～63.0 μg/mL(r=0.999 7)和0.120～24.0 μg/mL(r=0.999 9),平均回收率(n=3)分别为100.7，和99.9，,相对标准偏差(RSD)分别为2.11，和2.12，,检出限分别为42 ng/mL和43 ng/mL,样品中咖啡酸和阿魏酸的平均含量分别为3.26μg/mL和2.36μg/mL.
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关键词:
- 高效液相色谱,阿魏酸,咖啡酸,口服液
Abstract: An HPLC method was established for the simultaneous determination of ferulic acid and caffeic acid in oral liquid. The Shim-pack VP-ODS column (150 mm×4.6 mm, 0.3 μm) was used with a mobile were detected at a wavelength of 321 nm. The flow rate was 1.0 mL/min and the injection volume was 20 μL. The calibration curve was linear in the range of 0. 126-63.0 μg/mL (r=0. 999 7) for caffeic acid and 0. 120-24.0 μg/mL (r=0. 999 9) for ferulic acid. The average recovery (n=3) was 100.7% with RSD 2. 11% for caffeic acid and was 99.9% with RSD 2.12% for ferulic acid. The detection limit was 42 ng/mL for caffeic acid and 43 ng/mL for ferulic acid. The average content of the samples was 3. 26 μg/mL for caffeic acid and 2.36 μg/mL for ferulic acid. -
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