RP-HPLC测定藏药六味能消胶囊中土木香内酯和异土木香内酯的含量
Determination of Alantolactone and Isoalantolactone in the Tibetan Madicine Liuwei Nengxiao Capsule by RP-HPLC
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摘要: 目的:建立六味能消胶囊中土木香内酯和异土木香内酯的高效液相色谱法含量测定方法.方法:色谱柱为Kromasil C_(18)色谱柱(250 mm×4.6 mm,5μm);流动相为乙腈-0.1，磷酸溶液(58∶42);流速为1.0 mL/min;检测波长:194 nm.结果:土木香内酯、异土木香内酯均在0.30～1.50μg范围内呈良好的线性关系;r均为0.999 8;平均回收率分别为98.2，(RSD=1.67，,n=5)和97.7，(RSD=1.48，,n=5).结论:该方法简便、灵敏、准确,可作为六味能消胶囊的质量控制方法.Abstract: Objective To establish the HPLC method for the determination of alantolactone and isoalantolactone in Liuwei Nengxiao Capsule. Method ODS column (250 mm×4. 6 mm, 5 μm) of Kromasil; the mobile phase containing a mixture of acetonitril -0.05％ phosphoric acid solution (58 : 42); flow rate 1.0 mL·min~(-1); and the wavelength as 194 nm, were utilized for this study. Results Alantolactone and isoalantolactone showed good correlation in the ranges of 0. 30～1.5 μg (r=0. 999 8). The average recovery was 98.2% for alantolactone (RSD=1. 67%, n=5) and 97.7% for isoalantolactone (RSD=1. 48%, n=5). Conclusion The method is simple, accurate and sensitive and can be used to control the quality of Liuwei Nengxiao Capsule in its production.
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