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3种有益菌剂对烟草抗青枯病及根际微生物功能多样性的影响

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刘晓姣, 麻子君, 薛乐, 等. 3种有益菌剂对烟草抗青枯病及根际微生物功能多样性的影响[J]. 植物医学, 2021, (4): 23-30. doi: 10.13718/j.cnki.zwys.2021.04.006
引用本文: 刘晓姣, 麻子君, 薛乐, 等. 3种有益菌剂对烟草抗青枯病及根际微生物功能多样性的影响[J]. 植物医学, 2021, (4): 23-30. doi: 10.13718/j.cnki.zwys.2021.04.006
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LIU Xiaojiao, MA Zijun, XUE Le, et al. Effects of Adding Beneficial Microbial Agents in the Field on Tobacco Disease Resistance and Rhizosphere Microbial Diversity[J]. PLANT HEALTH AND MEDICINE, 2021, (4): 23-30. doi: 10.13718/j.cnki.zwys.2021.04.006
Citation: LIU Xiaojiao, MA Zijun, XUE Le, et al. Effects of Adding Beneficial Microbial Agents in the Field on Tobacco Disease Resistance and Rhizosphere Microbial Diversity[J]. PLANT HEALTH AND MEDICINE, 2021, (4): 23-30. doi: 10.13718/j.cnki.zwys.2021.04.006
shu

3种有益菌剂对烟草抗青枯病及根际微生物功能多样性的影响

  • 1. 西南大学 植物保护学院, 重庆 400715;

  • 2. 重庆市永川区种子推广站, 重庆 402160

详细信息

Effects of Adding Beneficial Microbial Agents in the Field on Tobacco Disease Resistance and Rhizosphere Microbial Diversity

  • 1. School of Plant Protection, Southwest University, Chongqing 400715, China;

  • 2. Seed Promotion Station of Yongchuan District, Chongqing 402160, China

  • 摘要: 土传青枯病是世界性防治难题，可以给烟草生产造成毁灭性损失.目前，田间防控烟草青枯病尚无有效的化学药剂，利用有益菌剂进行生物防治被视为一种可持续的防控措施.在本研究中，通过在大田中增施枯草芽孢杆菌（Bacillus subtilis，BS）、多粘类芽孢杆菌（Paenibacillus polymyxa，PP）和荧光假单胞菌（Pseudomonas fluorescens，PF）等有益菌剂，系统分析其对烟草生长、抗青枯病和根际微生物多样性的影响.结果表明，在本试验条件下，田间增施这3种菌剂均能提高烟草对青枯病的抗性，抗病效果表现为：PF>BS>PP；仅荧光假单胞菌剂能提高烟草团棵期的生长.对根际土壤微生物进行Biolog ECO测定，表明荧光假单胞菌剂处理对烟草根际微生物群落的均一性和碳源代谢能力有显著提升.对青枯菌具有诱集作用的4-羟基苯甲酸是荧光假单胞菌剂处理和枯草芽孢杆菌剂处理共同大量利用的碳源.本研究结果可为大田应用有益菌剂防治烟草青枯病提供重要的科学依据.
    Abstract: Soil-borne bacterial wilt is a worldwide problem, which causes devastating losses in tobacco production. At present, there is no effective chemical agent to control this disease, and thus the use of beneficial microbial agents for biological control is regarded as a sustainable prevention and control measure.A field experiment was conducted in which Bacillus subtilis (BS), Paenibacillus polymyxa (PP) and Pseudomonas fluorescens (PF) were applied as beneficial microbial agents and the growth of the tobacco plants, their resistance to bacterial wilt and the diversity of rhizosphere microbe were systematically analyzed.The results showed that the disease resistance of tobacco to bacteria wilt was improved by applying any of the three agents, the resistance effect being PF>BS>PP, but only the treatment of P. fluorescens improved the growth of tobacco at the rosette stage. Biolog ECO test showed that the treatment of P. fluorescens significantly improved the microbial evenness and carbon metabolic ability. 4-Hydroxy benzoic acid, which is supposed to be able to attract Ralstonia solanacearum, is the carbon source used in large quantities by the treatment of P. fluorescens and B. subtilis.
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  • [1] BERENDSEN R L, PIETERSE C M J, BAKKERP A H M. The Rhizosphere Microbiome and Plant Health[J]. Trends in Plant Science, 2012, 17(8):478-486.
    [2] JIANG G, WEI Z, XU J, et al. Bacterial Wilt in China:History, Current Status, and Future Perspectives[J]. Front Plant Sci, 2017, 8:1549.
    [3] LECOMTE C, DE ALABOUVETTE C, EDEL-HERMANN V, et al. Biological Control of Ornamental Plant Diseases Caused by Fusarium Oxysporum:a Review[J]. Biological Control, 2016, 101:17-30.
    [4] CHAPARRO J M, SHEFLIN A M, MANTER D K, et al. Manipulating the Soil Microbiome to Increase Soil Health and Plant Fertility[J]. Biology and Fertility of Soils, 2012, 48(5):489-499.
    [5] FRAVEL D R. Commercialization and Implementation of Biocontrol[J]. Annual Review of Phytopathology, 2005, 43(1):337-359.
    [6] 刘晓姣. 烟草根际抑病土壤有益微生物的组学特征及对青枯病的拮抗作用研究[D]. 重庆:西南大学, 2018.
    [7] 夏飞. 生防枯草芽孢杆菌、荧光假单胞杆菌及哈茨木霉菌对西瓜根围土壤细菌群落影响规律的研究[D]. 上海:华东理工大学, 2012.
    [8] KIELY P D, HAYNES J M, HIGGINS C H, et al. Exploiting New Systems-Based Strategies to Elucidate Plant-Bacterial Interactions in the Rhizosphere[J]. Microbial Ecology, 2006, 51(3):257-266.
    [9] 慕小倩, 杨超, 王硕. 荧光假单胞杆菌化感作用的初步研究[J]. 应用生态学报, 2005, 16(4):778-779.
    [10] RAMESH R, PHADKE G S. Rhizosphere and Endophytic Bacteria for the Suppression of Eggplant Wilt Caused by Ralstonia Solanacearum[J]. Crop Protection, 2012, 37:35-41.
    [11] 柳辉林, 张剑, 徐隆根, 等. 荧光假单胞菌3000亿个/克粉剂防治烟草青枯病田间药效试验[J]. 农药科学与管理, 2008, 29(6):24-26.
    [12] 刘艳霞, 李想, 蔡刘体, 等. 烟草根系分泌物酚酸类物质的鉴定及其对根际微生物的影响[J]. 植物营养与肥料学报, 2016, 22(2):418-428.
    [13] 李石力. 有机酸类根系分泌物影响烟草青枯病发生的机制研究[D]. 重庆:西南大学, 2017.
    [14] 王博. 外源酚酸对贝达葡萄植株生长及根际土壤微生物作用机制研究[D]. 沈阳:沈阳农业大学, 2016.
    [15] HASEGAWA T, KATO Y, OKABE A, et al. Effect of Secondary Metabolites of Tomato (Solanum lycopersicum) on Chemotaxis of Ralstonia solanacearum, Pathogen of Bacterial Wilt Disease[J]. Journal of Agricultural and Food Chemistry, 2019, 67(7):1807-1813.
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出版历程
  • 收稿日期:  2021-07-11

3种有益菌剂对烟草抗青枯病及根际微生物功能多样性的影响

    通讯作者: 丁伟, 主要从事烟草根茎病害防控研究.dingw@swu.edu.cn
    作者简介: 刘晓姣,博士,主要从事烟草根际土壤微生物研究.E-mail:xiaojiaoliu@email.swu.edu.cn
  • 1. 西南大学 植物保护学院, 重庆 400715;
  • 2. 重庆市永川区种子推广站, 重庆 402160
  • 收稿日期:  2021-07-11
基金项目: 

摘要: 土传青枯病是世界性防治难题，可以给烟草生产造成毁灭性损失.目前，田间防控烟草青枯病尚无有效的化学药剂，利用有益菌剂进行生物防治被视为一种可持续的防控措施.在本研究中，通过在大田中增施枯草芽孢杆菌（Bacillus subtilis，BS）、多粘类芽孢杆菌（Paenibacillus polymyxa，PP）和荧光假单胞菌（Pseudomonas fluorescens，PF）等有益菌剂，系统分析其对烟草生长、抗青枯病和根际微生物多样性的影响.结果表明，在本试验条件下，田间增施这3种菌剂均能提高烟草对青枯病的抗性，抗病效果表现为：PF>BS>PP；仅荧光假单胞菌剂能提高烟草团棵期的生长.对根际土壤微生物进行Biolog ECO测定，表明荧光假单胞菌剂处理对烟草根际微生物群落的均一性和碳源代谢能力有显著提升.对青枯菌具有诱集作用的4-羟基苯甲酸是荧光假单胞菌剂处理和枯草芽孢杆菌剂处理共同大量利用的碳源.本研究结果可为大田应用有益菌剂防治烟草青枯病提供重要的科学依据.

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