Effects of Different Application Methods of Pseudomonas fluorescens on the Occurrence of Tobacco Bacterial Wilt in Field
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摘要: 烟草青枯病在我国西南烟区普遍发生流行，是影响烟叶品质和产量的主要病害.为减轻烟草青枯病的危害，本研究以烤烟主栽品种"云烟87"为试验对象，通过田间小区试验探究荧光假单胞菌的不同施用方式对烟草青枯病发生以及对烟草生长发育的影响.结果表明，移栽期窝施5 g菌肥、100 mL微生物菌液灌根以及移栽期窝施5 g菌肥且灌根微生物菌液100 mL的最终防效分别为31.70%，27.35%和22.39，相互之间差异无统计学意义，且微生物菌剂处理区烟株长势优于对照区.综上所述，移栽时窝施菌肥或灌根菌液均对烟草青枯病具有一定的防效，研究结果为单菌种微生物菌剂的科学施用，推进烟草产业健康、绿色和可持续性发展提供理论基础.Abstract: Tobacco bacterial wilt is widespread in the tobacco-growing areas in southwestern China and is a main disease that affects the quality and yield of tobacco leaves. In order to reduce the harm imposed on tobacco crop by this disease and provide a theoretical basis for the scientific application of microbial agents and for the promotion of the healthy, green and sustainable development of the tobacco industry, a field experiment was made with Yunyan 87, the main variety of flue-cured tobacco, to explore the effects of different application methods of Pseudomonas fluorescens on the occurrence of tobacco bacterial wilt and the growth and development of tobacco. The results showed that application of the microbial (P. fluorescens) fertilizer at 5 g/hill during transplanting had the highest control efficiency on tobacco bacterial wilt, with an ultimate control efficiency of 31.70%, and root irrigation with 100 mL of the microbial bacterial solution gave a control efficiency of 27.35%. The tobacco plants in the microbial inoculant treatment area grew more vigorously than those in the control area. In summary, hill application of bacterial fertilizer or root-dressing of bacterial liquid has certain control effects on tobacco bacterial wilt during transplanting.
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