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苹果炭疽叶枯病病原菌分离鉴定及防治药剂的室内测定

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吕昭龙, 李继平, 郑果, 等. 苹果炭疽叶枯病病原菌分离鉴定及防治药剂的室内测定[J]. 植物医学, 2023, (1): 34-43. doi: 10.13718/j.cnki.zwyx.2023.01.005
引用本文: 吕昭龙, 李继平, 郑果, 等. 苹果炭疽叶枯病病原菌分离鉴定及防治药剂的室内测定[J]. 植物医学, 2023, (1): 34-43. doi: 10.13718/j.cnki.zwyx.2023.01.005
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LÜ Zhao-long, LI Ji-ping, ZHENG Guo, et al. Isolation and Identification of Pathogen of Apple Glomerella Leaf Spot and Laboratory Determination of Control Agents[J]. PLANT HEALTH AND MEDICINE, 2023, (1): 34-43. doi: 10.13718/j.cnki.zwyx.2023.01.005
Citation: LÜ Zhao-long, LI Ji-ping, ZHENG Guo, et al. Isolation and Identification of Pathogen of Apple Glomerella Leaf Spot and Laboratory Determination of Control Agents[J]. PLANT HEALTH AND MEDICINE, 2023, (1): 34-43. doi: 10.13718/j.cnki.zwyx.2023.01.005
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苹果炭疽叶枯病病原菌分离鉴定及防治药剂的室内测定

  • 1. 甘肃省农业科学院 植物保护研究所, 兰州 730070;

  • 2. 甘肃农业大学 植物保护学院, 兰州 730070

详细信息
    作者简介:

    吕昭龙,硕士研究生,主要从事植物病害及其综合防治研究. .

  • 中图分类号: S432

Isolation and Identification of Pathogen of Apple Glomerella Leaf Spot and Laboratory Determination of Control Agents

  • 1. Institute of Plant Protection, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China;

  • 2. College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, China

  • 摘要: 本研究对采自甘肃省礼县的苹果炭疽叶枯病病原菌种类进行了鉴定,并开展了防治药剂的室内筛选.通过组织分离法将病原菌分离纯化后,采用科赫氏法则进行致病性验证,结合形态学特征和内转录间隔区基因(Internal Transcribed Spacer,ITS)、 β-微管蛋白基因(β-Tubulin,TUB2)和肌动蛋白基因(Actin,ACT)多基因的系统发育分析对病原菌进行鉴定;并利用菌丝生长速率法测定6种化学杀菌剂对病原菌的抑制效果.结果表明,分离菌株为隐秘刺盘孢(Colletotrichum aenigma),可同时侵染叶片和果实,为当地苹果炭疽叶枯病致病菌.室内毒力测定结果表明,杀菌剂丙森锌和嘧菌环胺对菌丝的生长抑制效果较好,其EC50值分别为0.698 0 mg/L和1.232 6 mg/L;抑霉唑和异菌脲抑菌效果次之,EC50值分别为3.688 9 mg/L和2.684 0 mg/L;氰霜唑和氟啶胺抑菌效果较差,EC50值分别为58.740 9 mg/L和33.647 7 mg/L.研究结果为苹果炭疽叶枯病的有效防治提供了参考依据.
    Abstract: The purpose of this study was to determine the species of apple glomerella leaf spot in Li City, Gansu Province and to screen the control fungicides. In this study, the leaves suspected of being infected with apple glomerella leaf spot were used for isolation of the pathogen by tissue isolation method. The pathogenicity was verified by Koch's rule. The phylogenetic analysis was performed with Internal Transcribed Spacer(ITS), the β-tubulin gene(TUB2), and the Actin gene(ACT) to identify the pathogen in combining with the morphological characteristics. The inhibitory effect of 6 kinds of chemical fungicides on isolates was determined by mycelial growth rate method. The results showed that the isolate was Colletotrichumaenigma.Its pathogenicity showed that it could infect not only leaves but also fruits. The results of laboratory toxicity test showed that the Cyprodinil and Propineb had better inhibitory effect on the growth of mycelia with EC50 value of 0.698 0 mg/L and 1.232 6 mg/L, respectively. The effects of Imazalil and Iprodione were less with EC50 value of 3.688 9 mg/L and 2.684 0 mg/L, respectively. The effects of Cyazofamid and Fluazinam were the least with EC50 value of 58.940 9 mg/L and 33.647 7 mg/L, respectively. This study provides a reference for the prevention and treatment of apple glomerella leaf spot.
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  • [1] 刘克均.中国农业百科全书植物病理学卷[M].北京;农业出版社,1996.
    [2] 田香琳.果生刺盘孢交配型基因M477-2-1的功能分析 [D].杨凌:西北农林科技大学,2017.
    [3] 张俊祥,王美玉,迟福梅,等.苹果炭疽叶枯病菌CgCMK1基因的克隆与功能分析 [J].植物病理学报,2020,50(1):40-48.
    [4] LEITE RP,TSUNETA M,KISHINO AY.Ocorrência de Mancha Foliar de Glomerellaem Macieira no Estado do Paraná.Curitiba[M].Iapar:Informe de Pesquisa,1988.
    [5] WANGC X,ZHANGZ F,LIB H,et al.First Report of GlomerellaLeaf Spot of Apple Caused by Glomerella Cingulata in China [J].Plant Disease,2012,96(6):912.
    [6] 党建美,胡清玉,张瑜,等.炭疽菌叶枯病在我国苹果产区的发生分布及趋势分析 [J].北方园艺,2014(10):177-179.
    [7] ARAƯJOL, GONÇALVESAE, STADNIKM J. Ulvan Effect on Conidial Germination and Appressoria Formation of Colletotrichum gloeosporioides [J].Phytoparasitica,2014,42(5):631-640.
    [8] 吴建圆,王娜,冀志蕊,等.苹果炭疽叶枯病菌致病力分析及苹果种质抗病性鉴定 [J].植物遗传资源学报,2017,18(2):210-216.
    [9] 李保华,王彩霞,董向丽,等.苹果叶部三种主要病害的识别与防治 [J].果农之友,2016(5):30-31.
    [10] 李保华,王彩霞,董向丽.我国苹果主要病害研究进展与病害防治中的问题 [J].植物保护,2013,39(5):46-54.
    [11] 符丹丹.中国苹果炭疽病病原菌的遗传多样性 [D].杨凌:西北农林科技大学,2014.
    [12] 渭婷玉.果生刺盘孢MAPK信号通路Pmk1基因的功能分析 [D].杨凌:西北农林科技大学,2017.
    [13] 王晓鸣,李建义.陕西省刺盘孢的研究[J].真菌学报,1987,6(4):211-218.
    [14] 张荣,王素芳,崔静秋,等.陕、 豫两省苹果炭疽病病原鉴定 [J].中国农业科学,2009,42(9):3224-3229.
    [15] 李兰.代森锰锌与百菌清复配剂对苹果炭疽病的防治效果及对采后果实品质的影响 [D].杨凌:西北农林科技大学,2015.
    [16] 柴宝山,刘长令,李志念.嘧菌环胺的合成与杀菌活性 [J].农药,2007,46(6):377-378.
    [17] 韩文启,孙厚行,刘馨蔚,等.7种防治苹果炭疽叶枯病药剂的效果试验 [J].落叶果树,2018,50(6):49-51.
    [18] 常翠莲,渠非,冯浩,等.9种生物源杀菌剂对苹果炭疽叶枯病菌的室内活性评价 [J].南方农业,2020,14(15):133-134.
    [19] 王冰,王彩霞,史祥鹏,等.不同杀菌剂对苹果炭疽叶枯病的防治效果 [J].植物保护,2014,40(6):176-180.
    [20] 陈功友,张传伟,吴金成,史济华,张善侠,吴书方.苹果炭疽病菌对多菌灵抗药性研究 [J].果树科学,1993,10(3):150-153.
    [21] 王薇.苹果炭疽叶枯病病原学及苹果炭疽病侵染来源研究 [D].杨凌:西北农林科技大学,2017.
    [22] WHITET J,BRUNS T,LEE S,et al.Amplification and Direct Sequencing of Fungal Ribosomal RNA Genes for Phylogenetics[M]//PCR Protocols.Amsterdam:Elsevier,1990:315-322.
    [23] CARBONE I,KOHNL M.A Method for Designing Primer Sets for Speciation Studies in Filamentous ascomycetes [J].Mycologia,1999,91(3):553-556.
    [24] GLASSN L,DONALDSONG C.Development of Primer Sets Designed for Use with the PCR to Amplify Conserved Genes from Filamentous ascomycetes [J].Applied and Environmental Microbiology,1995,61(4):1323-1330.
    [25] DAMM U,CANNON P F,WOUDENBERG J H C,et al.The Colletotrichum boninense Species Complex [J].Studies in Mycology,2012,73:1-36.
    [26] 马永强.甘肃省苹果炭疽病病原鉴定及ITS序列分析 [J].江苏农业科学,2017,45(10):89-91.
    [27] 刘淼.戊唑醇与丙森锌混用对苹果斑点落叶病菌的联合毒力测定及最佳配比筛选 [J].农业与技术,2019,39(9):14-15,21.
    [28] VELHO A C.New Insights into the Characterization of Colletotrichum Species Associated with Apple Diseases in Southern Brazil and Uruguay [J].Fungal Biology,2015,119(4):229-244.
    [29] WALLHEAD M,BRODERS G,BEAUDOIN E,et al.Phylogenetic Assessment of Colletotrichum Species Associated with Bitter Rot and Glomerella Leaf Spot in the Northeastern US[J].Phytopathology,2014,104(11):123-124.
    [30] PICCIRILLO G,CARRIERI R,POLIZZI G,et al.In Vitro and in Vivo Activity of QoI Fungicides Against Colletotrichum gloeosporioides Causing Fruit Anthracnose in Citrus Sinensis [J].Scientia Horticulturae,2018,236:90-95.
    [31] 戴蓬博,张荣,孙广宇.中国苹果病害病原菌物名录 [J].菌物学报,2021,40(4):936-964.
    [32] 王薇,符丹丹,张荣,等.苹果炭疽叶枯病病原学研究 [J].菌物学报,2015,34(1):13-25.
    [33] WATCHANOK NAKLUMPA.中国苹果炭疽叶枯病菌种类多样性研究[D].杨凌:西北农林科技大学,2019.
    [34] 王美玉,冀志蕊,王娜,等.苹果炭疽叶枯病菌对3种杀菌剂的敏感性分析 [J].果树学报,2018,35(4):458-468.
    [35] 何艳维,宋梁栋,豆粉婷,等.关于苹果炭疽叶枯病防治药剂筛选研究 [J].陕西农业科学,2018,64(6):38-39.
    [36] 刘召阳,胡建邦,黄丽丽,等.6种杀菌剂对苹果炭疽叶枯病菌的室内活性评价 [J].乡村科技,2020,11(32):94-95.
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  • 收稿日期:  2022-11-20

苹果炭疽叶枯病病原菌分离鉴定及防治药剂的室内测定

    作者简介: 吕昭龙,硕士研究生,主要从事植物病害及其综合防治研究.
  • 1. 甘肃省农业科学院 植物保护研究所, 兰州 730070;
  • 2. 甘肃农业大学 植物保护学院, 兰州 730070
  • 收稿日期:  2022-11-20
基金项目: 

摘要: 本研究对采自甘肃省礼县的苹果炭疽叶枯病病原菌种类进行了鉴定,并开展了防治药剂的室内筛选.通过组织分离法将病原菌分离纯化后,采用科赫氏法则进行致病性验证,结合形态学特征和内转录间隔区基因(Internal Transcribed Spacer,ITS)、 β-微管蛋白基因(β-Tubulin,TUB2)和肌动蛋白基因(Actin,ACT)多基因的系统发育分析对病原菌进行鉴定;并利用菌丝生长速率法测定6种化学杀菌剂对病原菌的抑制效果.结果表明,分离菌株为隐秘刺盘孢(Colletotrichum aenigma),可同时侵染叶片和果实,为当地苹果炭疽叶枯病致病菌.室内毒力测定结果表明,杀菌剂丙森锌和嘧菌环胺对菌丝的生长抑制效果较好,其EC50值分别为0.698 0 mg/L和1.232 6 mg/L;抑霉唑和异菌脲抑菌效果次之,EC50值分别为3.688 9 mg/L和2.684 0 mg/L;氰霜唑和氟啶胺抑菌效果较差,EC50值分别为58.740 9 mg/L和33.647 7 mg/L.研究结果为苹果炭疽叶枯病的有效防治提供了参考依据.

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