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2015 Volume 37 Issue 9
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GUAN Ping,ZHANG Yu-jing,SHI Jian-ming,CHEN Ye. ISSR Analysis of the Genetic Diversity of the Endangered Plant Davidia involucrate Baill[J]. Journal of Southwest University Natural Science Edition, 2015, 37(9). doi: 10.13718/j.cnki.xdzk.2015.09.011
Citation: GUAN Ping,ZHANG Yu-jing,SHI Jian-ming,CHEN Ye. ISSR Analysis of the Genetic Diversity of the Endangered Plant Davidia involucrate Baill[J]. Journal of Southwest University Natural Science Edition, 2015, 37(9). doi: 10.13718/j.cnki.xdzk.2015.09.011
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ISSR Analysis of the Genetic Diversity of the Endangered Plant Davidia involucrate Baill

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  • ISSR (inter‐simple sequence repeat) markers were used to analyze the genetic diversity of 6 pop‐ulations of Davidia involucrate Baill and 1 population of D. involucrata Baill. var. vilmoriniana (Dode) Wanger ,the samples totaling 117 .A total of 199 discernible DNA loci were generated by 12 ISSR primers , of which 177 were polymorphic .At the species level ,percentage of polymorphic loci (PPL) ,genetic diver‐sity (He) and Shannon information index (I) was 88.94% ,0.278 4 and 0.418 7 ,respectively Polymor‐phism at the population level was rather low ,PPL ranging from 35.18% to 48.74% ,He from 0.126 0 to 0.179 5 ,and I from 0.188 1 to 0.265 1 .The genetic differentiation (Gst) based on the Nei's genetic diver‐sity was 0.474 5 ,indicating that there was 47.45 % of the variance among them and 52.55 % of the vari‐ance within them ,and the gene flow was 0.553 7 among them .Cluster analysis showed that there was no significant correlation between genetic distance and geographic distance .
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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ISSR Analysis of the Genetic Diversity of the Endangered Plant Davidia involucrate Baill

Abstract: ISSR (inter‐simple sequence repeat) markers were used to analyze the genetic diversity of 6 pop‐ulations of Davidia involucrate Baill and 1 population of D. involucrata Baill. var. vilmoriniana (Dode) Wanger ,the samples totaling 117 .A total of 199 discernible DNA loci were generated by 12 ISSR primers , of which 177 were polymorphic .At the species level ,percentage of polymorphic loci (PPL) ,genetic diver‐sity (He) and Shannon information index (I) was 88.94% ,0.278 4 and 0.418 7 ,respectively Polymor‐phism at the population level was rather low ,PPL ranging from 35.18% to 48.74% ,He from 0.126 0 to 0.179 5 ,and I from 0.188 1 to 0.265 1 .The genetic differentiation (Gst) based on the Nei's genetic diver‐sity was 0.474 5 ,indicating that there was 47.45 % of the variance among them and 52.55 % of the vari‐ance within them ,and the gene flow was 0.553 7 among them .Cluster analysis showed that there was no significant correlation between genetic distance and geographic distance .

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