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2021 Volume 34 Issue 4
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LIU Xiaojiao, MA Zijun, XUE Le, et al. Effects of Adding Beneficial Microbial Agents in the Field on Tobacco Disease Resistance and Rhizosphere Microbial Diversity[J]. PLANT HEALTH AND MEDICINE, 2021, (4): 23-30. doi: 10.13718/j.cnki.zwys.2021.04.006
Citation: LIU Xiaojiao, MA Zijun, XUE Le, et al. Effects of Adding Beneficial Microbial Agents in the Field on Tobacco Disease Resistance and Rhizosphere Microbial Diversity[J]. PLANT HEALTH AND MEDICINE, 2021, (4): 23-30. doi: 10.13718/j.cnki.zwys.2021.04.006
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Effects of Adding Beneficial Microbial Agents in the Field on Tobacco Disease Resistance and Rhizosphere Microbial Diversity

  • 1. School of Plant Protection, Southwest University, Chongqing 400715, China;

  • 2. Seed Promotion Station of Yongchuan District, Chongqing 402160, China

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  • Soil-borne bacterial wilt is a worldwide problem, which causes devastating losses in tobacco production. At present, there is no effective chemical agent to control this disease, and thus the use of beneficial microbial agents for biological control is regarded as a sustainable prevention and control measure.A field experiment was conducted in which Bacillus subtilis (BS), Paenibacillus polymyxa (PP) and Pseudomonas fluorescens (PF) were applied as beneficial microbial agents and the growth of the tobacco plants, their resistance to bacterial wilt and the diversity of rhizosphere microbe were systematically analyzed.The results showed that the disease resistance of tobacco to bacteria wilt was improved by applying any of the three agents, the resistance effect being PF>BS>PP, but only the treatment of P. fluorescens improved the growth of tobacco at the rosette stage. Biolog ECO test showed that the treatment of P. fluorescens significantly improved the microbial evenness and carbon metabolic ability. 4-Hydroxy benzoic acid, which is supposed to be able to attract Ralstonia solanacearum, is the carbon source used in large quantities by the treatment of P. fluorescens and B. subtilis.
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Effects of Adding Beneficial Microbial Agents in the Field on Tobacco Disease Resistance and Rhizosphere Microbial Diversity

  • Received Date: 11/07/2021

Abstract: Soil-borne bacterial wilt is a worldwide problem, which causes devastating losses in tobacco production. At present, there is no effective chemical agent to control this disease, and thus the use of beneficial microbial agents for biological control is regarded as a sustainable prevention and control measure.A field experiment was conducted in which Bacillus subtilis (BS), Paenibacillus polymyxa (PP) and Pseudomonas fluorescens (PF) were applied as beneficial microbial agents and the growth of the tobacco plants, their resistance to bacterial wilt and the diversity of rhizosphere microbe were systematically analyzed.The results showed that the disease resistance of tobacco to bacteria wilt was improved by applying any of the three agents, the resistance effect being PF>BS>PP, but only the treatment of P. fluorescens improved the growth of tobacco at the rosette stage. Biolog ECO test showed that the treatment of P. fluorescens significantly improved the microbial evenness and carbon metabolic ability. 4-Hydroxy benzoic acid, which is supposed to be able to attract Ralstonia solanacearum, is the carbon source used in large quantities by the treatment of P. fluorescens and B. subtilis.

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