Isolation and Identification of Pathogen of Apple Glomerella Leaf Spot and Laboratory Determination of Control Agents

LÜ Zhao-long; LI Ji-ping; ZHENG Guo; HUI Na-na; WANG Sen-shan; MA Sheng-biao; WANG Li

doi:10.13718/j.cnki.zwyx.2023.01.005

2023 Volume 2 Issue 1

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LÜ Zhao-long, LI Ji-ping, ZHENG Guo, et al. Isolation and Identification of Pathogen of Apple Glomerella Leaf Spot and Laboratory Determination of Control Agents[J]. PLANT HEALTH AND MEDICINE, 2023, (1): 34-43. doi: 10.13718/j.cnki.zwyx.2023.01.005

Citation:

LÜ Zhao-long, LI Ji-ping, ZHENG Guo, et al. Isolation and Identification of Pathogen of Apple Glomerella Leaf Spot and Laboratory Determination of Control Agents[J]. PLANT HEALTH AND MEDICINE, 2023, (1): 34-43. doi: 10.13718/j.cnki.zwyx.2023.01.005

Isolation and Identification of Pathogen of Apple Glomerella Leaf Spot and Laboratory Determination of Control Agents

1. Institute of Plant Protection, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China;
2. College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, China

More Information

Received Date: 20/11/2022
MSC: S432

Abstract

The purpose of this study was to determine the species of apple glomerella leaf spot in Li City, Gansu Province and to screen the control fungicides. In this study, the leaves suspected of being infected with apple glomerella leaf spot were used for isolation of the pathogen by tissue isolation method. The pathogenicity was verified by Koch's rule. The phylogenetic analysis was performed with Internal Transcribed Spacer(ITS), the β-tubulin gene(TUB2), and the Actin gene(ACT) to identify the pathogen in combining with the morphological characteristics. The inhibitory effect of 6 kinds of chemical fungicides on isolates was determined by mycelial growth rate method. The results showed that the isolate was Colletotrichumaenigma.Its pathogenicity showed that it could infect not only leaves but also fruits. The results of laboratory toxicity test showed that the Cyprodinil and Propineb had better inhibitory effect on the growth of mycelia with EC₅₀ value of 0.698 0 mg/L and 1.232 6 mg/L, respectively. The effects of Imazalil and Iprodione were less with EC₅₀ value of 3.688 9 mg/L and 2.684 0 mg/L, respectively. The effects of Cyazofamid and Fluazinam were the least with EC₅₀ value of 58.940 9 mg/L and 33.647 7 mg/L, respectively. This study provides a reference for the prevention and treatment of apple glomerella leaf spot.
- apple glomerella leaf spot,
- Colletotrichumaenigma,
- isolation and identification,
- determination of toxicity

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沈阳化工大学材料科学与工程学院沈阳 110142

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Isolation and Identification of Pathogen of Apple Glomerella Leaf Spot and Laboratory Determination of Control Agents

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