Cloning and Sequencing Analysis of the Readthrough Protein Gene of Different Tobacco Vein Distorting Virus Isolates of Yunnan Province

YANG Yahui; YANG Jie; CHEN Ping; CHEN Hairu; CHEN Xiaojiao; LI Fan

doi:10.13718/j.cnki.zwyx.2024.02.005

2024 Volume 3 Issue 2

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YANG Yahui, YANG Jie, CHEN Ping, et al. Cloning and Sequencing Analysis of the Readthrough Protein Gene of Different Tobacco Vein Distorting Virus Isolates of Yunnan Province[J]. PLANT HEALTH AND MEDICINE, 2024, (2): 38-48. doi: 10.13718/j.cnki.zwyx.2024.02.005

Citation:

YANG Yahui, YANG Jie, CHEN Ping, et al. Cloning and Sequencing Analysis of the Readthrough Protein Gene of Different Tobacco Vein Distorting Virus Isolates of Yunnan Province[J]. PLANT HEALTH AND MEDICINE, 2024, (2): 38-48. doi: 10.13718/j.cnki.zwyx.2024.02.005

Cloning and Sequencing Analysis of the Readthrough Protein Gene of Different Tobacco Vein Distorting Virus Isolates of Yunnan Province

College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China

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Received Date: 19/12/2023
MSC: S435.72

Abstract

In order to explore the variationsinthe readthrough protein gene (rtp gene) sequencesamong isolates of tobacco vein distorting virus (TVDV) collected at different times and from various regions of Yunnan Province， and analyze the factors contributing to the recent deceleration of tobacco bush top diseasein the field， the rtp gene sequences of TVDV was cloned and analyzed from the diseased tobacco samples collected from Chuxiong， Dali， Baoshan and HongheAutonomous Prefectures/City in Yunnan Province from 2020 to 2022. The rtp gene sequences of seven different TVDV isolates were obtained， all of them were 2 172 nucleotide (nt) in length， consisting of a 618-ntcp gene and 1 554-ntrtd domain. Consequently， the TVDV RTP protein has a total of 722 amino acids (aa)， comprising 205 aa of CP protein and 517 aa of RTD. Multiple alignment analysis results showed that the nt sequence identities of the cp genebetween the seven TVDV isolates obtained in this study and the TVDV isolates registered in GenBank were 98.71%～100%. Similarly， the aa sequence identities of the CP protein among different TVDV isolates were 98.05%～100%， with four aavariations. The nt sequence identities of the rtddomain between the seven TVDV isolates obtained in this study and the TVDV isolates derived fromGenBankranged from 96.68% to 99.81%. Likewise， the aa sequence identities of their RTD domain were 96.52%～100%， with thirty-nine aa differences.The variation of rtp gene in these TVDV isolates may affect the efficiency of aphid transmission of TVDV， and thus affect the occurrence and prevalence of tobacco bush top disease in the field.
- tobacco vein distorting virus,
- tobacco bushy top disease,
- readthrough protein,
- molecular variation,
- aphid transmission

References

[1]	MO X H， CHEN Z B， CHEN J P. Complete Nucleotide Sequence and Genome Organization of a Chinese Isolate of Tobacco Vein Distorting Virus [J]. Virus Genes， 2010， 41(3):425-431. Google Scholar
[2]	MO X H， CHEN Z B， CHEN J P. Molecular Identification and Phylogenetic Analysis of a Viral RNA Associated with the Chinese Tobacco Bushy Top Disease Complex [J]. Annals of Applied Biology， 2011， 158(2):188-193. Google Scholar
[3]	尹朝先，包娜，冉志伟，等. TBTV卫星RNA不同分离物的序列测定及系统进化分析 [J]. 云南农业大学学报(自然科学版)， 2010， 25(6):797-801. Google Scholar
[4]	MO X H， QIN X Y， TAN Z X， et al. First Report of Tobacco Bushy Top Disease in China [J]. Plant Disease， 2002， 86(1):74. Google Scholar
[5]	CHEN X J， LUO H M， ZHANG J Y， et al. Synergism among the Four Tobacco Bushy Top Disease Casual Agents in Symptom Induction and Aphid Transmission [J]. Frontiers in Microbiology， 2022， 13:846857. Google Scholar
[6]	李凡，吴建宇，陈海如. 烟草丛顶病研究进展 [J]. 植物病理学报， 2005， 35(5):385-391. Google Scholar
[7]	GATES L F. A Virus Causing Axillary Bud Sprouting of Tobacco in Rhodesia and Nyasaland [J]. Annals of Applied Biology， 1962， 50(1):169-174. Google Scholar
[8]	XU Y， JU H J， DEBLASIO S， et al. A Stem-Loop Structure in Potato Leafroll Virus Open Reading Frame 5 (ORF5) is Essential for Readthrough Translation of the Coat Protein ORF Stop Codon 700 Bases Upstream [J]. Journal of Virology， 2018， 92(11):15-17. Google Scholar
[9]	BRAULT V， VAN DEN HEUVEL J F， VERBEEK M， et al. Aphid Transmission of Beet Western Yellows LuteovirusRequires the Minor Capsid Read-through Protein P74 [J]. The EMBO Journal， 1995， 14(4):650-659. Google Scholar
[10]	LUCAS G B. Diseases of Tobacco[J]. Quarterly Review of Biology， 1965， 45(48):1421. Google Scholar
[11]	BRAULT V， HERRBACH ?， REINBOLD C. Electron Microscopy Studies on Luteovirid Transmission by Aphids [J]. Micron， 2007， 38(3):302-312. Google Scholar
[12]	BRAULT V， MUTTERER J， SCHEIDECKER D， et al. Effects of Point Mutations in the Readthrough Domain of the Beet Western Yellows Virus Minor Capsid Protein on Virus Accumulation in Planta and on Transmission by Aphids [J]. Journal of Virology， 2000， 74(3):1140-1148. Google Scholar
[13]	BRUYōRE A， BRAULT V， ZIEGLER-GRAFF V， et al. Effects of Mutations in the Beet Western Yellows Virus Readthrough Protein on Its Expression and Packaging and on Virus Accumulation， Symptoms， and Aphid Transmission [J]. Virology， 1997， 230(2):323-334. Google Scholar
[14]	BRAULT V， BERGDOLL M， MUTTERER J， et al. Effects of Point Mutations in the Major Capsid Protein of Beet Western Yellows Virus on Capsid Formation， Virus Accumulation， and Aphid Transmission [J]. Journal of Virology， 2003， 77(5):3247-3256. Google Scholar
[15]	CHAY C A， GUNASINGE U B， DINESH-KUMAR S P， et al. Aphid Transmission and Systemic Plant Infection Determinants of Barley Yellow Dwarf Luteovirus-PAV are Contained in the Coat Protein Readthrough Domain and 17-kDa Protein， Respectively [J]. Virology， 1996， 219(1):57-65. Google Scholar
[16]	LUCAS W J. Plant Viral Movement Proteins:Agents for Cell-to-Cell Trafficking of Viral Genomes [J]. Virology， 2006， 344(1):169-184. Google Scholar
[17]	LI R， MOCK R， HUANG Q， et al. A Reliable and Inexpensive Method of Nucleic Acid Extraction for the PCR-Based Detection of Diverse Plant Pathogens [J]. Journal of Virological Methods， 2008， 154(1-2):48-55. Google Scholar
[18]	魏太云，林含新，谢联辉. 植物病毒分子群体遗传学研究进展 [J]. 福建农业大学学报， 2003， 32(4):453-457. Google Scholar
[19]	GARCÍA-ARENAL F， FRAILE A， MALPICA J M. Variation and Evolution of Plant Virus Populations [J]. International Microbiology， 2003， 6(4):225-232. Google Scholar
[20]	TAN S T， LIU F， LV J， et al. Identification of Two Novel Poleroviruses and the Occurrence of Tobacco Bushy Top Disease Causal Agents in Natural Plants [J]. Scientific Reports， 2021， 11:21045. Google Scholar
[21]	LI Y Y， LIU Q L， XIANG D， et al. First Report of Natural Infection of Tobacco Bushy Top Virus on Tomato and Pepper in China [J]. Plant Disease， 2018， 102(7):1466. Google Scholar
[22]	杨馨，孟鈺，李梅蓉，等. 云南三七病毒病的发生及病毒种类检测 [J]. 植物病理学报， 2019， 49(4):456-464. Google Scholar
[23]	马普权，刘芳，谭冠林，等. 云南烟草丛顶病复合病原物的寄主范围 [J]. 云南农业大学学报(自然科学)， 2015， 30(3):363-368. Google Scholar
[24]	ZHAO W， XU Z T， ZHANG X M， et al. Genomic Variations in the 3’-Termini of Rice Stripe Virus in the Rotation between Vector Insect and Host Plant [J]. New Phytologist， 2018， 219(3):1085-1096. Google Scholar
[25]	YANG X L， WANG B， LUAN J B， et al. Molecular Variation of Tomato Yellow Leaf Curl Virus in the Insect Vector BemisiaTabaci [J]. Scientific Reports， 2017， 7:16427. Google Scholar
[26]	ROOSSINCK M J. Mechanisms of Plantvirus Evolution [J]. Annual Review of Phytopathology， 1997， 35:191-209. Google Scholar
[27]	BEACHY R N， LOESCH-FRIES S， TUMER N E. Coat Protein-Mediated Resistance Against Virus Infection [J]. Annual Review of Phytopathology， 1990， 28:451-472. Google Scholar
[28]	GADHAVE K R， GAUTAM S， RASMUSSEN D A， et al. Aphid Transmission of Potyvirus:The Largest Plant-Infecting RNA Virus Genus [J]. Viruses， 2020， 12(7):773. Google Scholar
[29]	PETER K A， GILDOW F， PALUKAITIS P， et al. The C Terminus of the Polerovirus P5 Readthrough Domain Limits Virus Infection to the Phloem [J]. Journal of Virology， 2009， 83(11):5419-5429. Google Scholar
[30]	杨程. 云南烟区烟草病虫害的立体防治 [J]. 植物医学， 2022， 1(3):18-24. Google Scholar
[31]	熊锋，吴维，毛倩卓，等. 利用免疫荧光标记技术分析TVDV在介体蚜虫消化道内的分布 [J]. 云南农业大学学报(自然科学)， 2018， 33(2):208-212. Google Scholar
[32]	张振甲，王德亚，于成明，等. 2种类型的烟草扭脉病毒外壳蛋白基因的序列和结构分析 [J]. 植物病理学报， 2016， 46(2):241-246. Google Scholar

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Cloning and Sequencing Analysis of the Readthrough Protein Gene of Different Tobacco Vein Distorting Virus Isolates of Yunnan Province

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