Identification and Distribution of Main Viruses in Leguminous Vegetables in Yunnan

MENG Yu; XIAO Long; LI Yueyue; TAN Guanlin; CHEN Xiaojiao; TAN Mingzhu; LAN Pingxiu; LI Fan

doi:10.13718/j.cnki.zwyx.2022.05.006

2022 Volume 1 Issue 5

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MENG Yu, XIAO Long, LI Yueyue, et al. Identification and Distribution of Main Viruses in Leguminous Vegetables in Yunnan[J]. PLANT HEALTH AND MEDICINE, 2022, (5): 54-63. doi: 10.13718/j.cnki.zwyx.2022.05.006

Citation:

MENG Yu, XIAO Long, LI Yueyue, et al. Identification and Distribution of Main Viruses in Leguminous Vegetables in Yunnan[J]. PLANT HEALTH AND MEDICINE, 2022, (5): 54-63. doi: 10.13718/j.cnki.zwyx.2022.05.006

Identification and Distribution of Main Viruses in Leguminous Vegetables in Yunnan

1. College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China;
2. Modern Education Technology Center, Yunnan Agricultural University, Kunming 650201, China

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Received Date: 22/09/2022
MSC: S52

Abstract

Viral diseases were surveyed and the diseased samples of broad bean, pea, bean and cowpea were collected from different leguminous vegetable planting areas in Yunnan Province, and then the viruses of Tobacco Mosaic Virus(TMV), Pea Seed-borne Mosaic Virus(PSbMV), Bean Yellow Mosaic Virus(BYMV), Broad Bean Wilt Virus 2(BBWV2)and Tomato Spotted Wilt Orthotospovirus(TSWV) commonly reported on leguminous vegetables were detected by RT-PCR. The main symptoms of leguminous vegetable viral diseases were necrosis, mosaic, malformation and leaf roll in broad bean; mosaic, and mostly with yellowing, necrosis, leaf roll, malformation in pea; mosaic and shrinking in bean; mottling, shrinking and vein yellowing in cowpea. Virus detection results showed that PSbMV, BBWV2 and TSWV were detected in pea samples with the detection ratio of 44.74%, 1.32% and 1.32%, respectively. BYMV, BBWV2 and PSbMV were detected in broad bean samples at 39.42%, 9.62% and 8.65% of detection rate, respectively. The 4.81% of the broad bean plants were co-infected with PSbMV and BYMV, and 8.57%, 1.43% and 1.43% of the bean samples were infected with CMV, PSbMV and BYMV, respectively. Only TSWV was detected in cowpea plants with 9.38% of detection rate. The virus diseases of leguminous vegetables were mainly distributed in Kunming, Chuxiong, Yuxi and Honghe. Samples from Kunming were infected with BBWV2, PSbMV, BYMV and CMV, from Yuxi were infected with BBWV2, PSbMV and TSWV, from Honghe were infected with BBWV2 and PSbMV, and from Chuxiong were infected with BYMV and PSbMV. PSbMV, BYMV, CMV, BBWV2 and TSWV are the main viruses infecting pea, broad bean, bean and cowpea, among which PSbMV and BYMV are the dominant viruses in Yunnan. PSbMV is the most widely distributed virus in Yunnan Province with relatively high detection rate.
- leguminous vegetable,
- virus disease,
- pathogen identification,
- Pea seed-borne mosaic virus,
- occurrence and distribution

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Identification and Distribution of Main Viruses in Leguminous Vegetables in Yunnan

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