引用本文:耿天佑, 罗理勇, 曾亮, 王兴华, 段永政.不同提取方法对沉香精油制备的影响[J].西南师范大学学报(自然科学版),2020,45(2):59~67
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不同提取方法对沉香精油制备的影响
耿天佑, 罗理勇, 曾亮, 王兴华, 段永政1,2,3
1. 西南大学 食品科学学院, 重庆 400715;2. 云南省普洱市茶叶科学研究所, 云南 普洱 665000;3. 云南西双版纳华夏沉香开发有限公司, 云南 西双版纳 666100
摘要:
以西双版纳州蚂蚁结香的沉香木为原料,通过水浴回流提取法、索氏提取法、超声辅助萃取法和微波辅助萃取法制备沉香精油,采用气相色谱-质谱联用法(GC-MS)分析精油成分,根据精油得率、成分种类和组成、特征性组分质量浓度比较4种提取方法对沉香精油制备的影响.结果表明,4种提取方法制备的精油成分组成相似,主要有倍半萜类、芳香族类、色酮类和脂肪酸类,但质量浓度存在较明显的差异;微波辅助萃取法制备沉香精油的提取率最高、物质种类最多,分别为1.32%和70种,其中反映沉香精油品质的倍半萜类物质和芳香族类物质的种类和质量浓度都高于其他3种方法,分别为19种、20种和22.29%,12.36%,且脂肪酸类物质的质量浓度最低,为24.30%;超声辅助萃取法所得精油中色酮类物质质量浓度最高,为38.62%,微波辅助萃取法次之,为36.52%;仅有微波辅助萃取法得到的精油中6种特征性物质均有检出,其中沉香螺醇、愈创木醇、苍术醇、(+)-香橙烯、γ-桉叶醇的质量浓度最高,分别为1.53%,1.73%,0.19%,1.47%和1.69%.综上所述,微波辅助萃取法是4种方法中最优的沉香精油提取方法,具有提取率高、精油品质佳的特点.
关键词:  沉香精油  提取方法  气质联用
DOI:10.13718/j.cnki.xsxb.2020.02.009
分类号:R283
基金项目:重庆市农业农村委员会项目(2019[6]);云南省科学技术厅"万人计划"产业技术领军人才项目(2019HB089).
On Effect of Different Extraction Methods on Preparation of Agarwood Essential Oil
GENG Tian-you, LUO Li-yong, ZENG Liang, WANG Xing-hua, DUAN Yong-zheng1,2,3
1. College of Food Science, Southwest University, Chongqing 400715, China;2. Tea Research of Pu'er Municipality, Pu'er yunnan 665000, China;3. Huaxia Aloes Development Limited Company, Xishuangbanna yunnan 666100, China
Abstract:
Agarwood essential oil was prepared by water bath eflux extraction, soxhlet extraction, ultrasonic-assisted extraction and microwave-assisted extraction with a sort of artificial agarwood damaged by ants in Xishuangbanna. The components of samples were measured by means of gas chromatography-mass spectrometry (GC-MC). Four types of agarwood essential oil extraction methods were studied comparatively on the basis of the yield, number and constituent of compounds, content of characteristic components of agarwood essential oil. The results show that the components of agarwood essential oil prepared by the four extraction methods were similar, mainly including sesquiterpenoids, aromatics, chromones and fatty acids, but the contents were significantly different. The extraction rate of agarwood essential oil obtained by microwave-assisted extraction was the highest, 1.32%, and species were the most 70. The kinds of sesquiterpenoids and aromatics on behalf of the quality of agarwood essential oil were 19 and 20 respectively, significantly more than the other three methods. The contents of sesquiterpenoids and aromatics in the agarwood essential oil prepared by microwave-assisted were the highest, 22.29% and 12.36% respectively, while the content of fatty acids was the lowest, 24.30%. The content of chromones in agarwood essential oil prepared by ultrasonic-assisted extraction was the highest, 38.62%, followed by microwave-assisted extraction, 36.52%. Six kinds of agarwood characteristic substances were only detected in essential oil obtained by microwave-assisted extraction, among which the contents of agarospirol, (-)-guaiol, atractylodin, (+)-aromadendrene and γ-eudesmol were the most, respectively 1.53%, 1.73%, 0.19%, 1.47% and 1.69%. All of these suggested that microwave-assisted extraction was the optimal extraction method for agarwood essential oil in the four methods, with high extraction yield and good quality.
Key words:  agarwood essential oil  extraction methods  gas chromatography-mass spectrometry (GC-MC)
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