引用本文:李卓姣[1] 、李丽[2].菱叶菊组培繁殖研究[J].西南师范大学学报(自然科学版),2017,42(8):
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菱叶菊组培繁殖研究
李卓姣[1] 、李丽[2]
作者单位
李卓姣[1] 、李丽[2] 西南大学 园艺园林学院,重庆,400715 重庆市南山植物园,重庆,400065 
摘要:
以菱叶菊(Chrysanthemumrhombifolium)幼嫩茎尖为材料,采用不同方法进行表面消毒后,以茎段和叶片为外植体,通过不定芽的诱导、继代和生根建立离体繁殖体系.结果表明,幼嫩茎尖的表面消毒以洗涤剂浸泡10min,自来水冲洗20min,75%乙醇消毒10s,0.1%HgCl2消毒5min的效果最好,成活率为75.15%.最适不定芽诱导培养基为MS+6-BA(3mg/L)+NAA(1mg/L),茎段的不定芽诱导率为82.2%,叶片的不定芽诱导率为66.6%;最适不定芽继代培养基为MS+6-BA(3mg/L)+NAA(1mg/L),增殖系数为4.63;最适不定芽生根培养基为1/2MS+IBA(0.5mg/L),生根率达98%.120株试管苗移栽到蚯蚓土和腐殖土(V(蚯蚓土)∶V(腐殖土)=1∶1)营养钵中,30d成活109株,移栽成活率达90.83%.
关键词:  菱叶菊  组织培养  快繁技术
DOI:10.13718/j.cnki.xsxb.2017.08.009
分类号:S682.1+1
基金项目:
Studies on Tissue Culture and Rapid Propagation of Chrysanthemum rhombifolium
LI Zhuo-jiao[1],LI Li[2]
Abstract:
Using the stem tips and young roots of Chrysanthemum rhombifolium as explants, in vitro propagation system was established by adventitious bud induction, subculture and rooting.The results showed that the surface disinfection of the young shoots was 10 min, the rinse with tap water for 20 min, 75% ethanol disinfection for 10 s, and 0.1% HgCl2 for 5 min.The survival rate was 75.15%.The optimum medium for adventitious bud induction was MS+6-BA(3 mg/L)+NAA(1 mg/L), the adventitious bud induction rate of stem tips was 82.2%, and the adventitious bud induction rate of leaves was 66.6%.The optimum medium for subculture of adventitious bud was MS+6-BA(3 mg/L)+NAA(1 mg/L), and the proliferation rate was 4.63.The optimum medium for bud rooting was 1/2 MS+IBA(0.5 mg/L), and the rooting rate was 98%.120 strains of test tube seedlings were transplanted into earthworm soil and humus soil (V(earthworm soil)∶V(humus soil)=1∶1) nutrition bowl, 30 days survived 109, transplant survival rate of 90.83%.
Key words:  Chrysanthemum rhombifolium  tissue culture  rapid propagation technique
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