引用本文:李琳琳, 刘佳, 苏贝贝, 杨卫星, 吴能表.茉莉酸甲酯对颠茄毛状根的生理指标及托品烷类生物碱积累的影响[J].西南大学学报(自然科学版),2017,39(5):70~75
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茉莉酸甲酯对颠茄毛状根的生理指标及托品烷类生物碱积累的影响
李琳琳, 刘佳, 苏贝贝, 杨卫星, 吴能表
西南大学 生命科学学院/三峡库区生态环境教育部重点实验室,重庆 400715
摘要:
该文研究茉莉酸甲酯(methyl jasmonate,MJ)对颠茄毛状根的逆境生理指标及莨菪碱、东莨菪碱含量的影响.结果表明,MJ的加入对颠茄毛状根生长具有一定的抑制作用,MDA、脯氨酸、可溶性蛋白质含量均有不同程度的变化:增加或先增加后降低;3种保护酶(SOD,POD,CAT)活性变化与诱导浓度有关;30 μmol/L的MJ处理组东莨菪碱含量最高,10 μmol/L的MJ处理组莨菪碱含量最高.表明MJ能显著促进颠茄毛状根中生物碱成分的积累.
关键词:  茉莉酸甲酯  颠茄  毛状根  东莨菪碱  莨菪碱  
DOI:10.13718/j.cnki.xdzk.2017.05.011
分类号:
基金项目:
Effects of Methyl Jasmonate on Physiological Indexes and Accumulation of Main Tropane Alkaloids in Atropa belladonna L. Hairy Roots
LI Lin-lin, LIU Jia, SU Bei-bei, YANG Wei-xing, WU Neng-biao
Abstract:
To study the effects of methyl jasmonate (MJ) on physiological indexes and accumulation of main tropane alkaloids in Atropa belladonna L. hairy roots, MJ was added in different concentrations to the medium. The results showed that MJ treatments inhibited hairy root growth, and the contents of soluble protein, MDA and pro increased in different extends or first increased and then decreased. Changes in the activity of the three protective enzymes (SOD, POD and CAT) was associated with the induction concentration. The highest accumulation of scopolamine and hyoscyamine was observed in the treatments of 30 μmol/L and 10 μmol/L MJ, respectively, thus indicating that MJ could significantly promote the accumulation of secondary metabolites in A. belladonna hairy roots.
Key words:  methyl jasmonate  Atropa belladonna  hairy root  scopolamine  hyoscyamine  
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