引用本文:张翠平,李琳琳,韦 悦,卢克欢,郭 双,吴能表.3 种常用培养基对颠茄毛状根生长与次生代谢产物积累的影响[J].西南大学学报(自然科学版),2017,39(6):36~41
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3 种常用培养基对颠茄毛状根生长与次生代谢产物积累的影响
张翠平,李琳琳,韦 悦,卢克欢,郭 双,吴能表
西南大学 生命科学学院/三峡库区生态环境教育部重点实验室,重庆 400715
摘要:
研究了B5,MS,N6 3种培养基对颠茄毛状根的生长及托品烷类生物碱质量分数和产量的影响.结果表明:B5,MS培养基中颠茄毛状根的生物量明显高于N6培养基,两者之间差异无统计学意义,但B5培养基中东莨菪碱和莨菪碱质量分数与产量均显著高于MS培养基.因此,比较3种培养基,B5培养基作为基本培养基不仅有利于颠茄毛状根的生长,也对托品烷类生物碱的合成有促进作用,为建立适合其生长和次生代谢产物产生的离体培养体系提供了理论依据.
关键词:  颠茄  毛状根  培养基  托品烷类生物碱  
DOI:10.13718/j.cnki.xdzk.2017.06.006
分类号:
基金项目:国家自然科学基金项目(30500041);重庆市科技攻关项目(cstc2012gg-yyjs80013)
Effects of Three Media on the Growth and Secondary Metabolite Contents of Atropa belladonna L. Hairy Roots
ZHANG Cui-ping, LI Lin-lin, WEI Yue, LU Ke-huan, GUO Shuang, WU Neng-biao
Abstract:
In order to provide a basis for establishing an in vitro culture system suitable for the growth and secondary metabolite production of Atropa belladonna L., a suspension culture was made in which the effects of medium types on the growth of A. belladonna hairy roots and TAs production in them were studied. The biomass of A. belladonna hairy roots in B5 and MS was significantly higher than in N6, with no significant difference between the two media. However, the content and production of scopolamine and hyoscyamine in B5 were significantly higher than in MS.These results showed that B5 was the best medium for hairy root growth of A. belladonna and for the accumulation of tropane alkaloids in it.
Key words:  Atropa belladonna L.  hairy root  medium  tropane alkaloids (TAs)  
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