引用本文:汪云叶, 童虹宇, 周小雪, 周启贵, 李娟, 汤绍虎.北碚榕组培不定芽的两段生根[J].西南大学学报(自然科学版),2018,40(12):46~50
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北碚榕组培不定芽的两段生根
汪云叶, 童虹宇, 周小雪, 周启贵, 李娟, 汤绍虎1,2
1. 西南大学 生命科学学院, 重庆 400715;2. 三峡库区生态环境教育部重点实验室, 重庆 400715
摘要:
以北碚榕组培不定芽为外植体,采用两段生根法生根(在琼脂培养基中诱导不定根发生,在近黑暗多孔隙基质中完成根系发育)并将其与一般生根法比较,培养30 d后统计2种方法的生根率,测定生根苗生长与生理指标;生根苗移栽30 d后统计移栽成活率,测定移栽苗生长指标.试验结果表明:二者生根率均为100%;与一般生根法相比,两段生根后生根苗的根尖数、总根长、根总体积和根平均直径分别提高301.42%,27.85%,123.53%和9.68%;生根苗的株高和叶片增量分别提高307.89%和83.33%,叶绿素含量和硝酸还原酶活性分别提高13.09%和297.29%;生根苗的移栽成活率(100%)、移栽苗的株高和叶片增量分别提高15.00%、41.12%和23.81%,且二者间差异有统计学意义.实验结果证明,两段生根法显著优于一般生根法.所得试管苗根系发达,移栽成活率更高,生长状态更佳.两段生根法对于其它植物的组培生根有应用价值.
关键词:  北碚榕  两段生根法  植物组织培养
DOI:10.13718/j.cnki.xdzk.2018.12.007
分类号:Q948
基金项目:重庆市林业重点科技攻关项目(渝林科研2016-4);国家自然科学基金项目(31370317).
Two-Stage Rooting of Adventitious Buds of Ficus beipeiensis in Tissue Culture
WANG Yun-ye, TONG Hong-yu, ZHOU Xiao-xue, ZHOU Qi-gui, LI Juan, TANG Shao-hu1,2
1. School of Life Sciences, Southwest University, Chongqing 400715, China;2. Key Laboratory of Eco-Environments in Three Gorges Reservoir Region, Ministry of Education, Chongqing 400715, China
Abstract:
The adventitious buds of Ficus beipeiensis were used as explants and a two-stage rooting method was used to generate roots (inducing adventitious roots in an agar medium and completing root development in a near-dark multi-porous matrix), and the results were compared with those of the general rooting method. The rooting rate was calculated and the growth and physiological indexes of the rooted plantlets were measured after they were cultured for 30 days. Their survival rate was counted and their growth indexes were measured 30 days after transplanting. The test results showed that the rooting rate of both methods was 100%. Compared with the general rooting method, the two-stage rooting method increased the number of root tips, total root length, total root volume and average root diameter of the rooted plantlets by 301.42%, 27.85%, 123.53% and 9.68%, respectively; enhanced plant height and leaf increment by 307.89% and 83.33%; and raised chlorophyll content and nitrate reductase activity 13.09% and 297.29%, respectively. All the rooted plantlets survived after transplanting, and the survival rate, plant height and leaf number were increased by 15.00%, 41.12% and 23.81%, respectively, and the differences between the two methods were statistically significant. In conclusion, the two-stage rooting method was significantly better than the general rooting method. The root system of the test-tube plantlets was well-developed, the survival rate after transplanting was higher, and the growth state was better. The two-stage rooting method has application value for the rooting of other plants in tissue culture.
Key words:  Ficus beipeiensis  two-stage rooting method  plant tissue culture
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