引用本文:何燕, 邓永辉, 李梦寒, 冯西博, 卓嘎.藜麦品系的染色体数目及核型分析[J].西南大学学报(自然科学版),2019,41(1):27~31
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藜麦品系的染色体数目及核型分析
何燕, 邓永辉, 李梦寒, 冯西博, 卓嘎1,2
1. 西藏农牧学院 植物科学学院, 西藏 林芝 860000;2. 西南大学 农学与生物科技学院, 重庆 400715
摘要:
为探明西藏目前主推藜麦的染色体数目及核型,以西藏农牧学院植物科学学院提供的藜麦品系W4为材料,对其进行根尖染色体常规压片法制片,比较采用8-羟基喹啉、秋水仙素和冰冻方法的预处理时间、1 mol/L HCl酸解时间对藜麦染色体制片的影响,探讨最优的根尖处理方法并进行核型分析.结果显示:用0.1%秋水仙素溶液(离体)处理3 h,1 mol/L HCl 60℃酸解13~14 min的总体作用效果最佳;藜麦W4的核型公式为2n=36=32 m(2SAT)+4 sm,核型不对称系数为57.87%,核型分类中属2B型.
关键词:  藜麦W4  染色体制片  核型分析
DOI:10.13718/j.cnki.xdzk.2019.01.004
分类号:S512
基金项目:西藏自治区重点项目(XZXTCX-2016).
Chromosome Number and Karyotype Analysis of Quinoa (Chenopodium quinoa Willd)
HE Yan, DENG Yong-hui, LI Meng-han, FENG Xi-bo, ZHUO Ga1,2
1. College of Plant Science, Xizang Agriculture and Animal Husbandry College, Linzhi Tibet 860000, China;2. School of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China
Abstract:
To investigate the chromosome number and karyotype of quinoa (Chenopodium quinoa Willd) cv. W4, the main line of the species in Tibet, its seeds provided by College of Plant Science of Xizang Agriculture and Animal Husbandry College were used as the plant material. The traditional chromosome tabletting technique was adopted and the effects of the time of pretreatment with 8-hydroxyquinoline and colchicine and with freezing and acidolysis time with 1 mol/L HCl on chromosome preparations were compared so as to explore the best method of root tip treatment and karyotype analysis. Treatment with 0.1% colchicine solution (in vitro) for 3 hours, and acidolysis with 1 mol/L HCl at 60℃ for 13~14 minutes gave the best results. The karyotype formula of quinoa W4 was shown to be 2n(Chenopodium quinoa Willd)=36=32 m(2SAT)+4 sm, with an asymmetrical coefficient of 57.87%. In classification, it belonged to the category of karyotype 2B.
Key words:  Chenopodium quinoa Willd W4  chromosome preparation  karyotype analysis
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