引用本文:刘云露, 郑守豪, 叶磊鑫, 廖国建, 胡昌华.黄连土壤阿魏酸降解菌的筛选及降解特性[J].西南大学学报(自然科学版),2019,41(1):46~50
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黄连土壤阿魏酸降解菌的筛选及降解特性
刘云露, 郑守豪, 叶磊鑫, 廖国建, 胡昌华
西南大学 药学院, 重庆 400716
摘要:
为筛选黄连土壤来源的阿魏酸降解菌,从黄连种植基地的土壤中分离能够在以阿魏酸为唯一碳源的培养基中生长的真菌,ITS序列比对鉴定种属,采用高效液相色谱法检测菌株在不同条件下对阿魏酸的降解能力.结果表明:获得对阿魏酸具有降解能力的真菌F-0056,鉴定为齿孢青霉(Penicillium daleae);外源阿魏酸添加量为200 μg/mL时,菌株F-0056培养72 h后阿魏酸降解率达87.5%;真菌F-0056在土壤环境下144 h对阿魏酸降解效率达71.8%.
关键词:  黄连  阿魏酸  降解菌  土壤微生物  连作障碍
DOI:10.13718/j.cnki.xdzk.2019.01.007
分类号:S567.5+2
基金项目:国家重点研发计划项目(2017YFC1702600);重庆市社会民生科技创新专项项目(cstc2016shmszx80102).
Isolation and Degradation Characteristics of Ferulic Acid-Degrading Fungi in Rhizosphere Soil of Coptis chinensis
LIU Yun-lu, ZHENG Shou-hao, YE Lei-xin, LIAO Guo-jian, HU Chang-hua
School of Pharmaceutical Science and Chinese Medicine, Southwest University, Chongqing 400716, China
Abstract:
In order to screen ferulic acid-degrading microbes from rhizosphere soil of Coptis chinensis,a fungus strain which could grow in a medium with ferulic acid as the sole carbon source was isolated from the soil of a C. chinensis plantation. With ITS (internal transcribed spacer) sequence analysis this strain (F-0056) was identified as Penicillium daleae. High performance liquid chromatography (HPLC) was used to determine its degradation ability of ferulic acid under different conditions,and the results showed that the ferulic acid degradation rate of F-0056 reached 87.5% after 72 hours' culture in a medium supplemented with ferulic acid at 200 μg/mL and 71.8% in a soil environment ofter 144 hours.
Key words:  Coptis chinensis  ferulic acid  degrading microbe  soil microorganism  continuous cropping obstacle
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