引用本文:刘兴, 张翠平, 杨怡, 韦悦, 吴能表.茉莉酸甲酯对颠茄生长及其氮代谢的影响[J].西南大学学报(自然科学版),2019,41(2):9~16
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茉莉酸甲酯对颠茄生长及其氮代谢的影响
刘兴, 张翠平, 杨怡, 韦悦, 吴能表1,2,3
1. 西南大学 生命科学学院/三峡库区生态环境教育部重点实验室, 重庆 400715;2. 沂水县第一中学, 山东 沂水 276400;3. 中国农业科学学院 棉花研究所, 新疆 昌吉 831100
摘要:
以颠茄(Atropa belladonna)幼苗为材料,研究了不同质量浓度(0,100,200,300和400 μmol/L)茉莉酸甲酯(Methyl Jasmonate,MeJA)处理不同时间(7,14,21和28 d)对颠茄鲜质量、光合色素和主要含氮化合物质量分数以及氮代谢关键酶活性的影响,为药材产量的提高提供理论依据.结果表明,随处理时间的延长,各质量浓度MeJA处理组中颠茄鲜质量、光合色素质量分数呈降低的趋势,并明显低于同时期对照(0 μmol/L);相反可溶性蛋白(SP)、游离脯氨酸(Pro)质量分数呈增加的趋势,其中低质量浓度(100,200 μmol/L)MeJA更能促进SPPro质量分数的积累.在MeJA处理28 d时,硝态氮质量分数显著降低(p<0.05);硝酸还原酶(GS)与谷氨酰胺合成酶(NR)活性的变化趋于一致,呈先升高后降低趋势,均是在200 μmol/L MeJA处理时活性达到最高值,分别是对照的1.35倍和1.672倍.综上,MeJA能够抑制颠茄的生长,降低光合色素、硝态氮质量分数,而低质量浓度MeJA可提高SPPro质量分数以及GSNR活性,因此在颠茄人工栽培过程中喷施低质量浓度MeJA有利于颠茄的生长和氮素代谢.
关键词:  颠茄  茉莉酸甲酯  生长  氮代谢  硝酸还原酶  谷氨酰胺合成酶
DOI:10.13718/j.cnki.xdzk.2019.02.002
分类号:S567
基金项目:国家自然科学基金项目(30500041).
Effect of Methyl Jasmonate on the Growth of Atropa belladonna and Its Nnitrogen Metabolism
LIU Xing, ZHANG Cui-ping, YANG Yi, WEI Yue, WU Neng-biao1,2,3
1. School of Life Science, Southwest University/Key Laboratory of Eco-Environments in Three Gorges Reservoir Region, Ministry of Education, Chongqing 400715, China;2. No.1 Middle School of Yishui, Yishui Shandong 276400, China;3. Cotton Research Institute, CAAS, Changji Xinjiang 831100, China
Abstract:
To offer a theoretical support for improving the yield of Atropa belladonna, we investigated the effects of different concentrations of methyl jasmonate (0, 100, 200, 300 and 400 μmol/L) over different time periods (7, 14, 21 and 28 d) on the fresh weight, chlorophyll, main nitrogenous compound contents and key enzyme activities in nitrogen metabolism of this Chinese traditional medicine crop. With the prolongation of treatment time, the fresh weight and the content of chlorophyll showed a descending trend in all MeJA treatment groups, which was significantly lower than those of the control group (0 μmol/L). In contrast, the content of soluble protein (SP) and proline (Pro) tended to increase, and the low concentration of MeJA (100 and 200 μmol/L) could promote the accumulation of SP and Pro. When treated with MeJA for 28 days, the nitrate content significantly decreased (p<0.05), and the activities of nitrate reductase (NR) and glutamine synthetase (GS) tended to decrease, which was the highest at 200 μmol/L MeJA, being 1.35 and 1.672 times that of the control group, respectively. In conclusion, MeJA can inhibit the growth of A. belladonna and reduce the content of chlorophyll and nitrate in it, and the low mass concentration of MeJA can increase the content of SP and Pro as well as the activities of GS and NR. Therefore, a low concentration of MeJA appears to be more beneficial to the growth and nitrogen metabolism in A. belladonna.
Key words:  Atropa belladonna  methyl jasmonate  growth  nitrogen metabolism  nitrate reductase  glutamine synthetase
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