引用本文:曾珍, 李玲, 杨帆, 郑一敏, 吉光见稚代, 胥秀英.牛蒡子配方颗粒制备与牛蒡子苷元质量分数测定研究[J].西南大学学报(自然科学版),2019,41(2):27~32
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牛蒡子配方颗粒制备与牛蒡子苷元质量分数测定研究
曾珍, 李玲, 杨帆, 郑一敏, 吉光见稚代, 胥秀英
重庆理工大学 药学与生物工程学院, 重庆 400054
摘要:
对牛蒡子配方颗粒制备工艺筛选及牛蒡子苷元质量分数进行测定,采用正交优化方法进行处方筛选,HPLC法测定牛蒡子苷元质量分数.色谱条件:色谱柱:Dionex C18(250 mm×4.6 mm,5 μm);流动相:甲醇与水的比例为40:60;流速:1.0 mL/min;检测波长:280 nm;柱温:室温.结果表明,牛蒡子颗粒的最佳制备工艺:物料比即牛蒡子提取物、糊精、淀粉比例为3:6:1,润湿剂:85%乙醇,干燥温度:65℃.在上述色谱条件下牛蒡子苷元在1.41 μg~7.05 μg范围内线性关系良好,r=0.999 9.方法回收率100.09%,RSD为2.19%.该方法可为牛蒡子相关制剂的开发和质量研究提供参考.
关键词:  牛蒡子  配方颗粒  正交实验  牛蒡子苷元
DOI:10.13718/j.cnki.xdzk.2019.02.004
分类号:Q949.783.5;R283.6
基金项目:重庆市科委重点项目(CSTC2014zkjccxyyBX0034).
Study on Preparation of Fructus arctii Formulated Granules and Determination of Arctigenin in Them
ZENG Zhen, LI Ling, YANG Fan, ZHENG Yi-min, YOSHIMITSU Michiyo, XU Xiu-ying
College of Pharmaceutical and Biological Engineering, Chongqing University of Technology, Chongqing 400054, China
Abstract:
To screen for the optimal preparation process of Fructus arctii formulated granules and to determine the arctigenin content in them. The orthogonal optimization method was used to screen the prescriptions and HPLC was performed to determine the arctigenin content. Analysis was carried out with Dionex C18 (250 mm×4.6 mm, 5 μm), the mobile phase was CH3OH-H2O (40:60), the flow rate was 1.0 mL/min, the wave length was 280 nm and the temperature was room temperature. The optimal preparation process of F. arctii granules was as follows:The ratio of F. arctii extract:dextrin:starch was 3:6:1, the wetting agent was 85% ethanol and the temperature was 65℃. Under these conditions, it had a good linear relationship, the average recovery of arctigenin was 100.09%, and RSD was 2.19%. The method described above can be used for the development and quality control of F. arctii preparations.
Key words:  Fructus arctii  formulated granule  orthogonal optimization  arctigenin
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