引用本文:顾偌铖, 唐运林, 吴燕燕, 牛小慧, 李青晏, 郭志斌, 向丽, 蒋睿轩, 雷云飞, 刘秀, 胡源, 包佳玲, 李田, 韦俊宏, 潘国庆, 周泽扬.重庆地区取食高粱的草地贪夜蛾与玉米粘虫肠道细菌比较[J].西南大学学报(自然科学版),2019,41(8):6~13
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重庆地区取食高粱的草地贪夜蛾与玉米粘虫肠道细菌比较
顾偌铖, 唐运林, 吴燕燕, 牛小慧, 李青晏, 郭志斌, 向丽, 蒋睿轩, 雷云飞, 刘秀, 胡源, 包佳玲, 李田, 韦俊宏, 潘国庆, 周泽扬1,2,3,4,5,6,7,8
1. 西南大学 家蚕基因组生物学国家重点实验室, 重庆 400715;2. 西南大学 农业部蚕桑生物学与遗传育种重点实验室, 重庆 400715;3. 西南大学 微孢子虫感染与防控重庆市重点实验室, 重庆 400715;4. 西南大学 生物技术学院, 重庆 400715;5. 重庆市种子管理站, 重庆 401121;6.6. 江津区农业技术推广中心, 重庆 江津 402260;7.7. 重庆江记农业科技有限公司, 重庆 江津 402289;8.8. 重庆师范大学 生命科学学院, 重庆 401331
摘要:
草地贪夜蛾(Spodoptera frugiperda)于2019年5月被发现已经入侵至重庆地区,6月底在江津地区的高粱地中也发现了少量的草地贪夜蛾,同时还发现了大量玉米粘虫(Mythimna seperata).通过分离培养同一生境内的草地贪夜蛾和玉米粘虫的肠道细菌,结合16S rDNA测序完成了这两种害虫肠道优势菌属水平的鉴定,共分离得到了19个细菌分离株,其中分离自草地贪夜蛾肠道细菌归为5个属,分别为欧文氏菌属(Erwinia),不动杆菌属(Acinetobacter),肠杆菌属(Enterobacter),肠球菌属(Enterococcus),假单胞菌属(Pseudomonas).分离自玉米粘虫肠道细菌归为6个属,分别为克雷伯氏菌属(Klebsiella),不动杆菌属(Acinetobacter),寡养单胞菌属(Stenotrophomonas),肠球菌属(Enterococcus),金黄杆菌属(Chryseobacterium),芽孢杆菌属(Bacillus).同时我们也对采自玉米地和高粱地的草地贪夜蛾肠道微生物优势菌进行了比较分析,在江津高粱地中的草地贪夜蛾中没有发现克雷伯氏菌,但该菌在巫山、巫溪玉米地的草地贪夜蛾中大量存在.结论为进一步研究草地贪夜蛾幼虫肠道核心微生物群奠定了基础.
关键词:  重庆地区  高粱  草地贪夜蛾  玉米粘虫  肠道细菌
DOI:10.13718/j.cnki.xdzk.2019.08.002
分类号:Q93-331
基金项目:中央高校基本科研业务费团队项目(XDJK2018AA001);家蚕基因组生物学国家重点实验室自设课题(2019-03).
Comparison of Gut Bacteria Between Spodoptera frugiperda and Mythimna seperata Feeding on Sorghum in Chongqing Area
顾偌铖, 唐运林, 吴燕燕, 牛小慧, 李青晏, 郭志斌, 向丽, 蒋睿轩, 雷云飞, 刘秀, 胡源, 包佳玲, 李田, 韦俊宏, 潘国庆, 周泽扬1,2,3,4,5,6,7,8
1. State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China;2. Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture, Chongqing 400715 China;3. Chongqing Key Laboratory of Microsporidia Infection and Control, Chongqing 400715, China;4. College of Biotechnology, Southwest University, Chongqing 400715, China;5. Crop Seed Management Station of Chongqing, Chongqing 401121, China;6.6. Jiangjin Agricultural Technology Extension, Jiangjin Chongqing 402260, China;7.7. Chongqing Jiangji Agricultural Technology Limited, Jiangjin Chongqing, 402289, China;8.8. College of Life Sciences, Chongqing Normal University, Chongqing 401331, China
Abstract:
In May of 2019, Spodoptera frugiperda migrated to Chongqing and spread rapidly. It was found in maize fields in several counties and districts. At the end of June, a little S. frugiperda and plenty Mythimna seperata were found in sorghum fields in Jiangjin district. In this study we isolated the intestinal dominant bacteria from the larvae of S. frugiperda and M. seperata, and used 16S rDNA sequencing to identify their dominant genera. In total, we obtained 19 isolates of bacteria, of which Erwinia, Acinetobacter, Enterobacter, Enterococcus and Pseudomonas were identified from S. frugiperda; Klebsiella, Acinetobacter, Stenotrophomonas, Enterococcus, Chryseobacterium and Bacillus were idenfified from M. seperata. The dominant bacteria types in the gut of the two insects in the same habitat were obviously diffierent although two genera were same. At the same time, we also conducted a comparative analysis of the dominant intestinal bacteria of S. frugiperda in the maize field and the sorghum field. We did not find Klebsiella sp. in S. frugiperda feeding on sorghum in Jiangjin district, which was abundant in the S. frugiperda feeding on maize in Wushan and Wuxi. This work may lay a foundation for further study on the core intestinal microorganisms of the S. frugiperda larvae.
Key words:  Chongqing area  sorghum  Spodoptera frugiperda  Mythimna seperata  gut bacteria
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