引用本文:吴燕燕, 耿敬可, 徐思, 唐运林, 顾偌铖, 韦俊宏, 潘国庆, 周泽扬.重庆地区草地贪夜蛾幼虫肠道真菌的分离鉴定[J].西南大学学报(自然科学版),2019,41(9):1~8
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重庆地区草地贪夜蛾幼虫肠道真菌的分离鉴定
吴燕燕, 耿敬可, 徐思, 唐运林, 顾偌铖, 韦俊宏, 潘国庆, 周泽扬1,2,3,4,5
1. 西南大学 家蚕基因组生物学国家重点实验室, 重庆 400715;2.
2. 西南大学 农业部蚕桑生物学与遗传育种重点实验室, 重庆 400715;3.
3. 西南大学 微孢子虫感染与防控重庆市重点实验室, 重庆 400715;4.
4. 西南大学 生物技术学院, 重庆 400715;5.
5. 重庆师范大学 生命科学学院, 重庆 401331
摘要:
草地贪夜蛾(Spodoptera frugiperda)是一种洲际迁飞性农业害虫,食谱广泛.2019年5月已迁飞至重庆地区,对农作物造成严重危害.为了解入侵重庆地区草地贪夜蛾(Spodoptera frugiperda)肠道真菌菌群的组成,本研究以采集自重庆巫山玉米田及重庆江津高粱田中的草地贪夜蛾幼虫为材料,运用传统培养方法分离其粪便中的肠道优势真菌,并对其进行形态学观察和分子生物学鉴定.实验通过分离培养不同地区、不同食物的草地贪夜蛾肠道真菌,结合形态学观察及rDNA ITS测序完成了属水平的鉴定,共分离得到了5个属10个真菌分离株,其中分离自重庆巫山玉米田的草地贪夜蛾肠道真菌归为3个属,分别为念珠菌属(Candida)、掷孢酵母属(Sporobolomyces)和帚枝霉属(Sarocladium);分离自重庆江津高粱田的草地贪夜蛾肠道真菌归为3个属,分别为念珠菌属(Candida)、莫氏黑粉菌属(Moesziomyces)和毛霉属(Mucor).初步分离获得入侵重庆地区的草地贪夜蛾肠道真菌,丰富了对入侵草地贪夜蛾肠道微生物的认识.
关键词:  重庆地区  草地贪夜蛾  肠道真菌  鉴定
DOI:10.13718/j.cnki.xdzk.2019.09.001
分类号:Q969.436.5;Q93-331
基金项目:中央高校基本科研业务费团队项目(XDJK2018AA001);家蚕基因组生物学国家重点实验室自设课题(2019-03).
Isolation and Identification of Intestinal Fungi in Spodoptera frugiperda Larvae of Chongqing Area
WU Yan-yan, GENG Jing-ke, XU Si, TANG Yun-lin, GU Ruo-cheng, WEI Jun-hong, PAN Guo-qing, ZHOU Ze-yang1,2,3,4,5
1. State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China;2.
2. Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture, Chongqing 400715, China;3.
3. Chongqing Key Laboratory of Microsporidia Infection and Control, Chongqing 400715, China;4.
4. School of Biotechnology, Southwest University, Chongqing 400715, China;5.
5. College of Life Sciences, Chongqing Normal University, Chongqing 401331, China
Abstract:
Spodoptera frugiperda is a migratory agricultural pest with a wide range of recipes. It migrated to Chongqing in May of 2019, causing serious damages to crops. In order to explore the structural composition of the intestinal fungal flora of S. frugiperda larvae in Chongqing, we used the larvae of S. frugiperda collected from the corn field in Wushan and the sorghum field in Jiangjin. The dominant intestinal fungi in the feces were isolated by traditional culture method and identified with morphological observation and ITS sequencing. In the experiments, ten fungal isolates were obtained and classified into five genera. The intestinal fungi isolated from the larvae in the Wushan corn field were classified into three genera, Candida, Sporobolomyces and Sarocladium. The intestinal fungi isolated from S. frugiperda of the Jiangjin sorghum field were classified into three genera, Candida, Moesziomyces and Mucor. This experiment initially isolated the intestinal fungi of S. frugiperda, thus enriching our understanding of the intestinal microbes of this pest.
Key words:  Chongqing area  Spodoptera frugiperda  intestinal fungus  identification
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