引用本文:滕左, 宋春红, 于杰.基于DNA宏条形码的莼菜大田土壤真菌多样性研究[J].西南大学学报(自然科学版),2019,41(9):52~58
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基于DNA宏条形码的莼菜大田土壤真菌多样性研究
滕左, 宋春红, 于杰1,2
1. 西南大学 园艺园林学院, 重庆 400716;2.
2. 中国烟草总公司重庆市公司彭水分公司, 重庆 彭水 409699
摘要:
高通量测序与DNA条形码结合产生的DNA宏条形码技术(DNA-Metabarcoding),能快速鉴定混合样本中的物种,现已成为检测群类物种多样性和丰富度的常用方法.本试验采用这一方法分析了莼菜大田不同种植年限土壤真菌多样性,结果表明:10年生土壤中含有最多的真菌种类,多样性及丰富度最高;在属水平上表现为蛙粪霉属、裂梗霉属、酵母属等占优势;群类组成分析显示,种植年限的变化对土壤真菌群落组成有一定的影响;聚类分析中10年与15年生莼菜土壤物种多样性相似度较高.
关键词:  种植年限  莼菜  DNA条形码  高通量测序  鉴定
DOI:10.13718/j.cnki.xdzk.2019.09.007
分类号:S154.3;Q949.91
基金项目:重庆市社会事业与民生保障科技创新专项项目(cstc2017shms-kjfp80009);西南大学石柱创新基金项目/校县合作项目(Sz201802).
A DNA-Metabarcoding-Based Study of the Diversity of Soil Fungi in the Brasenia schreberi Field
TENG Zuo, SONG Chun-hong, YU Jie1,2
1. Shool of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716, China;2.
2. China National Tobacco Corp(Pengshui Division), Pengshui Chongqing 409699, China
Abstract:
DNA-Metabarcoding, which is a combination of high-throughput sequencing and DNA barcoding, enables the rapid identification of species in mixed samples and has become a common method for detecting species diversity and abundance. In an experiment reported herein, this method was used to analyze the diversity of soil fungi in Brasenia schreberi J. F. Gmel fields with different cultivation years. The results indicated the soil on which B. schreberi had been planted for 10 years contained the most fungal species with the highest diversity and abundance. At the genus level, Trichoderma, Phyllospora and Saccharomyces were predominant. Group composition analysis showed that the change of the cultivation year had a certain influence on the composition of soil fungal communities. In the cluster analysis, the similarity of soil species diversity between the 10th and 15th years was higher, and they were clustered together. The results of this study may lay a theoretical foundation for the scientific cultivation of B. schreberi.
Key words:  cultivation year  Brasenia schreberi  DNA barcoding  high-throughput sequencing  identification
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