引用本文:张凤梅, 何明忠, 李可欣, 高杰, 韩志刚, 韩文莉, 潘永全, 衣启营.5种不同商品化的饲料对C57BL/6J和KM小鼠生长和繁殖的影响[J].西南大学学报(自然科学版),2019,41(9):59~67
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5种不同商品化的饲料对C57BL/6J和KM小鼠生长和繁殖的影响
张凤梅, 何明忠, 李可欣, 高杰, 韩志刚, 韩文莉, 潘永全, 衣启营
重庆医科大学 实验动物中心, 重庆 400016
摘要:
研究市场上常见的5种饲料对C57BL/6J小鼠及KM小鼠生长和繁殖的影响,为实验小鼠生产中合理使用饲料提供科学依据.实验选取3周龄C57BL/6J小鼠和KM小鼠各250只,每种鼠随机分为5组,每组50只,雌雄各半,饲喂A,B,C,D,E共5种不同厂家的实验动物维持饲料,测定小鼠4~8周龄的体质量和脏器系数.另选取C57BL/6J小鼠及KM小鼠性成熟小鼠各150只,雌雄各半,每种鼠随机分为5组,每组15对,饲喂A,B,C,D,E共5种不同厂家的实验动物繁殖饲料,测定母鼠的产仔数、连胎率.结果表明:① 5种维持饲料均能满足小鼠的生长需求,C饲料和E饲料更符合小鼠生长的营养需求.② 5种饲料饲喂的小鼠器官指数正常.③ 5种繁殖饲料饲喂的母鼠第1,2胎的产仔数差异不具有统计学意义(p>0.05),说明产仔数更多取决于遗传因素,本次调查的几种饲料对其影响较小.合理选择实验小鼠所需饲料,利于实验数据的稳定.
关键词:  饲料  生长  繁殖  C57BL/6J小鼠  KM小鼠
DOI:10.13718/j.cnki.xdzk.2019.09.008
分类号:Q95-331
基金项目:重庆市科委资助项目(cstc2015shmszx120099,cstc2015shmszx120017,cstc2015shmszxsydw).
Effects of Five Different Commercial Feeds on the Growth and Breeding of C57BL/6J and KM Mice
ZHANG Feng-mei, HE Ming-zhong, LI Ke-xin, GAO Jie, HAN Zhi-gang, HAN Wen-li, PAN Yong-quan, YI Qi-ying
Laboratory Animal Center, Chongqing Medical University, Chongqing 400016, China
Abstract:
In order to provide a scientific basis for rational use of feed in experimental mouse production, an experiment was conducted to investigate the effects of five commercial brands of feed on the growth and breeding of C57BL/6J and Kunming mice. Two hundred and fifty healthy 3-week-old C57BL/6J mice and 250 healthy 3-week-old Kunming mice were randomly divided into 5 groups, with 25 pairs in each (half in sex) and fed with feeds of different producers (A, B, C, D and E), respectively. The body weight and organ index of 4-8-week-old mice were determined. Again, 150 sexually mature C57BL/6J mice, half male and female, were randomly allocated into 5 groups, with 15 pairs in each, and fed with A, B, C, D and E. The number of pups and the fetal rate of the female mice were determined. A similar experiment was carried out with Kunming mice. The results showed that all the 5 kinds of maintenance feed could meet the growth needs of the mice, C and E being more satisfactory for the nutritional needs of the growth of the mice; that the mice of all the 5 groups showed normal organ index; and that there was no significant difference in pups between the first and second births of the female mice, which were fed with the five kinds of breeding feeds, thus suggesting that the number of pups depends more on genetic factors than on the feed. In summary, a proper choice of the feed of experimental mice will be conducive to the stability of experimental data.
Key words:  feed  growth  breed  C57BL/6J mouse  Kunming mouse (KM mouse)
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