引用本文:任明波, 杨毅, 刘杰, 刘春雷, 刘燕琴.南川百合资源现状及组培快繁技术研究[J].西南大学学报(自然科学版),2019,41(11):19~24
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南川百合资源现状及组培快繁技术研究
任明波, 杨毅, 刘杰, 刘春雷, 刘燕琴
重庆市药物种植研究所/特色生物资源研究与利用川渝共建重点实验室, 重庆 南川 408435
摘要:
研究发现,近年南川百合资源呈明显下降趋势,评估为易危(VU)等级.以鳞茎为外植体进行芽的诱导、增殖培养,筛选出培养配方:MS+6-BA 1.4 mg/L+NAA 0.2 mg/L+白糖30 g/L+琼脂7.0 g/L,pH=5.8;壮苗、生根培养基:MS+NAA 0.3 mg/L+香蕉泥15 g/L+活性炭0.1%+白糖40 g/L,pH=5.8;可以实现南川百合快繁和资源保护.
关键词:  南川百合  资源  组培  丛生芽诱导  壮苗培养
DOI:10.13718/j.cnki.xdzk.2019.11.003
分类号:Q949.71+8.23
基金项目:重庆市科委基本科研业务费计划项目(2016cstc-jbky-01315).
Study of the Resource Status of Lilium rosthornii Diels and Its Rapid Propagation Through Tissue Culture
REN Ming-bo, YANG Yi, LIU Jie, LIU Chun-lei, LIU Yan-qin
Chongqing Institute of Medicinal Plant Cultivation/Bio-resource Research and Utilization Joint Key laboratory of Sichuan and Chongqing, Nanchuan Chongqing 408435, China
Abstract:
It was found in a study reported in this paper that the resources of Lilium rosthornii Diels showed a significant downward trend in recent years and was evaluated as of the vulnerable grade (VU). Then, the bulbs of L. rosthornii were used as explants in tissue culture for bud induction and proliferation, and the best medium selected was MS+6-BA 1.4 mg/L+NAA 0.2 mg/L+sugar 30 g/L+agar 7.0 g/L at a pH of 5.8. The optimum medium for rooting was MS+NAA 0.3 mg/L+mashed banana 15 g/L+active carbon 0.1%+sugar 40 g/L at pH 5.8. With these methods, L. rosthornii rapid propagation and resource conservation can be realized.
Key words:  Lilium rosthornii Diels  resource  tissue culture  clumped bud induction  best seed cultivation
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