引用本文:李鹏佳, 焦茂娟, 王启军, 苏承刚.魔芋GDP甘露糖焦磷酸化酶基因及其启动子的克隆与分析[J].西南大学学报(自然科学版),2019,41(3):14~22
【打印本页】   【HTML】   【下载PDF全文】   查看/发表评论  【EndNote】   【RefMan】   【BibTex】
←前一篇|后一篇→ 过刊浏览    高级检索
本文已被:浏览 354次   下载 127 本文二维码信息
码上扫一扫!
分享到: 微信 更多
魔芋GDP甘露糖焦磷酸化酶基因及其启动子的克隆与分析
李鹏佳, 焦茂娟, 王启军, 苏承刚
西南大学 园艺园林学院/南方山地园艺学教育部重点实验室, 重庆 400715
摘要:
以花魔芋和白魔芋为材料,通过魔芋5个转录组高通量测序数据库,与拟南芥进行tBlastn比对、拼接,得到魔芋中的GDP-甘露糖焦磷酸化酶基因GMP的推定全长,设计基因特异性引物,克隆得到魔芋GMP基因的部分cDNA序列,长度为1 233 bp,其中基因编码区长度为1 086 bp,编码的氨基酸为361个.在此基础上运用FNPI-PCR技术得到了魔芋GDP-甘露糖焦磷酸化酶基因GMP上游启动子2 116 bp,经生物信息学分析,该序列具有典型的TATA-box、CAAT-box及与胁迫相关的元件和光应答元件.
关键词:  魔芋  GDP-甘露糖焦磷酸化酶  启动子克隆与功能分析
DOI:10.13718/j.cnki.xdzk.2019.03.003
分类号:S632.3
基金项目:国家级大学生创新创业训练计划项目(201710635018);国家自然科学基金项目(31071796).
Cloning and Characterization of GDP-Mannose Pyrophosphorylase Gene in Konjac and Its Promoter
LI Peng-jia, JIAO Mao-juan, WANG Qi-jun, SU Cheng-gang
School of Horticulture and Landscape Architecture, Southwest University/Key Laboratory of Horticulture Science for Southern Mountainous Regions of Ministry of Education, Chongqing 400715, China
Abstract:
In this study, tBlastn searches of high-throughput transcriptome sequencing databases of Amorphophallus konjac and A. albus were aligned and spliced with the amino acid sequence of Arabidopsis thaliana, and the GMP transcript was assembled. The putative full-length sequence of the GMP gene was cloned. By designing gene-specific primers, a partial cDNA sequence of konjac GMP gene with a length of 1 233 bp was cloned. The length of the coding region of the gene was 1086 bp, and the encoded amino acid was 361. Based on the above results, using FNPI-PCR technology, we got konjac GDP-mannose pyrophosphorylase gene GMP upstream promoter 2 116 bp in length. According to bioinformatics analysis, typical TATA-box, CAAT-box, and stress-related elements and light responsive elements and other cis-elements were identified in the promoter region.
Key words:  Amorphophallus  GDP-mannose pyrophosphorylase  promoter cloning and functional analysis
手机扫一扫看