引用本文:黄汉成, 江艾耘, 罗永莉, 杨晓迪, 李相兰, 胡志刚, 周荣琼.犬弓首蛔虫abcg-5基因的克隆及序列分析[J].西南大学学报(自然科学版),2019,41(3):23~29
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犬弓首蛔虫abcg-5基因的克隆及序列分析
黄汉成, 江艾耘, 罗永莉, 杨晓迪, 李相兰, 胡志刚, 周荣琼
西南大学 动物科学学院, 重庆 荣昌 402460
摘要:
研究犬弓首蛔虫(Toxocara canis,T.canis)腺苷三磷酸结合盒转运蛋白(ATP-binding cassette transporters,ABC)亚家族G5基因(abcg-5)的分子特征.根据犬弓首蛔虫基因组数据库中的Tc-abcg-5基因序列设计引物,通过PCR技术克隆Tc-abcg-5全长基因,并进行多重序列比对和种系发育进化树分析.结果表明:该基因全长为1 902 bp,编码633个氨基酸.功能结构域分析发现TcABCG5蛋白包含1个ABC转运蛋白结构域和6个跨膜区,同时发现了高度保守的Walker A和Walker B模体.GO分析显示ABCG5具有ATP结合和ATP酶活性.种系发育进化树分析显示TcABCG5与猪蛔虫进化关系较近,与哺乳动物进化关系较远.
关键词:  犬弓首蛔虫  abcg-5基因  克隆  序列分析
DOI:10.13718/j.cnki.xdzk.2019.03.004
分类号:S858.292
基金项目:国家自然科学基金项目(31672541;31172313);中央高校基本科研业务项目(XDJK2016E087;XDJK2017D080).
Cloning and Sequence Analysis of the Full-Length abcg-5 Gene of Toxocara canis
HUANG Han-cheng, JIANG Ai-yun, LUO Yong-li, YANG Xiao-di, LI Xiang-lan, HU Zhi-gang, ZHOU Rong-qiong
College of Animal Science, Southwest University, Rongchang Chongqing 402460, China
Abstract:
To study the molecular characteristics of ATP-binding cassette sub-family G member 5 (abcg-5) in Toxocara canis, the full-length coding sequence of Tc-abcg-5 gene was amplified using PCR amplification, multiple sequence alignment (MSA) was made and a phylogenetic tree was constructed. The results showed that the entire sequence of Tc-abcg-5 gene contained 1 902 bp, encoding a protein of 633 amino acids. TcABCG5 was found to contain one ABC transporter domain and six transmembrane regions. The highly conserved Walker A and Walker B motifs of the ABCG sub-family were also found. Gene ontology (GO) annotations showed that TcABCG5 had the molecular function of ATP binding and ATPase activity. Phylogenetic analysis indicated that TcABCG5 shared a high level of amino-acid sequence similarity with Ascaris suum, and shared a low level of amino-acid sequence similarity with mammals. This study is expected to lay a foundation for further functional researches of TcABCG5.
Key words:  Toxocara canis  ATP-binding cassette subfamily G member 5 (abcg-5) gene  cloning  sequence analysis
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