引用本文:万路, 黄玉明, 奉萍.基于NH2-MIL-101(Fe)过氧化物模拟酶特性测定葡萄糖[J].西南大学学报(自然科学版),2019,41(3):84~88
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基于NH2-MIL-101(Fe)过氧化物模拟酶特性测定葡萄糖
万路, 黄玉明, 奉萍
西南大学 化学化工学院, 重庆 400715
摘要:
研究发现NH2-MIL-101(Fe)金属有机框架材料可催化H2O2氧化3,3,5,5-四甲基联苯胺(TMB)显蓝色,表现出过氧化物模拟酶特性,而且在较宽的温度(4~80℃)及pH值范围(2~10)内保持其模拟酶活性,结合葡萄糖氧化酶,建立了测定葡萄糖的方法.在优化条件下,吸光度与葡萄糖浓度在0.75~50 μmol/L范围内呈现良好的线性关系,对葡萄糖的检出限为0.75 μmol/L.将本法用于血清中葡萄糖的测定,获得满意结果.
关键词:  NH2-MIL-101(Fe)  光度法  模拟酶  葡萄糖
DOI:10.13718/j.cnki.xdzk.2019.03.012
分类号:X703
基金项目:国家自然科学基金项目(51678485).
NH2-MIL-101(Fe) Mimetic Peroxidase-Based Determination of Glucose
WAN Lu, HUANG Yu-ming, FENG Ping
School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China
Abstract:
The material of NH2-MIL-101(Fe) metal-organic framework was found to be able to catalyze the oxidization of 3, 3', 5, 5'-tetramethylbenzidine (TMB) by H2O2 to produce a blue product, showing intrinsic peroxidase-like activity. Furthermore, the NH2-MIL-101(Fe) was found to exhibit good robustness in a wide temperature range from 4 to 80℃ and in a wide pH range from 2 to 10. In a study reported herein, a new method for the detection of glucose was developed when combined with glucose oxidase. Under optimal conditions, the absorbance at 652 nm showed linear response with glucose concentrations in the range from 0.75 μmol/L to 50 μmol/L with a detection limit of 0.75 μmol/L. The proposed method was applied to determine the content of glucose in human serum samples and satisfactory results were obtained.
Key words:  NH2-MIL-101(Fe)  spectrophotometry  mimetic enzyme  glucose
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